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手工天然发酵干香肠生产中肠衣对蛋白水解变化和生物胺含量的影响

Casing Contribution to Proteolytic Changes and Biogenic Amines Content in the Production of an Artisanal Naturally Fermented Dry Sausage.

作者信息

Serio Annalisa, Laika Jessica, Maggio Francesca, Sacchetti Giampiero, D'Alessandro Flavio, Rossi Chiara, Martuscelli Maria, Chaves-López Clemencia, Paparella Antonello

机构信息

Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, via Balzarini 1, 64100 Teramo, Italy.

出版信息

Foods. 2020 Sep 13;9(9):1286. doi: 10.3390/foods9091286.

Abstract

The effect of two kinds of casings on the production and characteristics of a dry fermented sausage was investigated. In detail, an Italian product, naturally fermented at low temperatures and normally wrapped in beef casing instead of the most diffused hog one, was selected. Two different productions (one traditionally in beef casing (MCB) and another in hog casing (MCH)) were investigated over time to determine the differences particularly regarding proteolytic changes during fermentation and ripening. First of all, the product in hog casing required a longer ripening time, up to 120 days, instead of 45-50 days, because of the lower drying rate, while the microbial dynamics were not significantly modified. Conversely, the proteolysis showed a different evolution, being more pronounced, together with the biogenic amines content up to 341 mg/Kg instead of 265 mg/Kg for the traditional products. The latter products were instead characterized by higher quantities of total free amino acids, 3-methyl butanoic acid, 3-Methyl-1-butanal, and 2-Methylpropanal, enriching the final taste and aroma. The traditional product MCB also showed lower hardness and chewiness than MCH. The results highlight how the choice of casing has a relevant impact on the development of the final characteristics of fermented sausages.

摘要

研究了两种肠衣对干发酵香肠生产及特性的影响。具体而言,选取了一种意大利产品,该产品在低温下自然发酵,通常用牛肠衣包裹,而非最常用的猪肠衣。对两种不同的生产方式(一种传统上使用牛肠衣(MCB),另一种使用猪肠衣(MCH))随时间进行了研究,以确定差异,特别是在发酵和成熟过程中蛋白水解变化方面的差异。首先,由于干燥速度较慢,使用猪肠衣的产品需要更长的成熟时间,长达120天,而不是45 - 50天,同时微生物动态没有显著改变。相反,蛋白水解呈现出不同的演变,更为明显,生物胺含量高达341毫克/千克,而传统产品为265毫克/千克。后一种产品的特征是总游离氨基酸、3 - 甲基丁酸、3 - 甲基 - 1 - 丁醛和2 - 甲基丙醛的含量更高,丰富了最终的味道和香气。传统产品MCB的硬度和咀嚼性也低于MCH。结果突出了肠衣的选择对发酵香肠最终特性的形成具有重要影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/477c/7554767/1f2224d832ba/foods-09-01286-g001.jpg

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