从“嘉宝果”（Plinia cauliflora）果皮中提取的一种富含半乳糖的杂多糖。
A galactose-rich heteropolysaccharide extracted from "jaboticaba" (Plinia cauliflora) peels.
机构信息
Setor de Engenharia de Alimentos, Escola de Agronomia, Universidade Federal de Goiás, Goiânia-GO, Brazil; Laboratório de Química de Polímeros, Instituto de Ciências Biológicas, ICB 2, Universidade Federal de Goiás, Goiânia-GO, Brazil.
Laboratório de Química de Polímeros, Instituto de Ciências Biológicas, ICB 2, Universidade Federal de Goiás, Goiânia-GO, Brazil; Laboratório de Ressonância Nuclear Magnética, Universidade Federal de São Carlos, São Carlos-SP, Brazil.
出版信息
Carbohydr Polym. 2020 Dec 1;249:116821. doi: 10.1016/j.carbpol.2020.116821. Epub 2020 Jul 30.
The objective of this work was to extract, identify and characterize a galactose-rich heteropolysaccharide (GH) from "jaboticaba" peel. The best conditions to extract the GH according to a 2 full-factorial experimental design were 90 °C/30 min/pH 1.0, resulting in a 32.32 % yield using lyophilized sample. The chemical structure analyzed by GC/MS and NMR spectra (HSQC/HSQC-TOCSY) showed that the main chain of GH consists of a (1→4) galactoside branched at carbon 3, containing galactose (67.21 %), glucose (15.78 %), arabinose (9.78 %), rhamnose (2.26 %) and traces of esterified and non-esterified uronic acids. Rheological studies revealed that GH suspensions behave as a Newtonian fluid, with calculated molecular mass of 1.48 × 10 Da. The absolute viscosity of 1 % (w/v) aqueous suspension of GH decreased from 25 mPa s to 10 mPa s in NaCl and 7 mPa s in CaCl, indicating the polyelectrolyte character of GH.
本工作旨在从“嘉宝果”果皮中提取、鉴定和表征一种富含半乳糖的杂多糖（GH）。根据 2 全因子实验设计，GH 的最佳提取条件为 90°C/30min/pH1.0，使用冻干样品时产率为 32.32%。通过 GC/MS 和 NMR 谱（HSQC/HSQC-TOCSY）分析的化学结构表明，GH 的主链由（1→4）半乳糖苷组成，在碳 3 处支化，含有半乳糖（67.21%）、葡萄糖（15.78%）、阿拉伯糖（9.78%）、鼠李糖（2.26%）和痕量酯化和非酯化的糖醛酸。流变学研究表明，GH 悬浮液表现为牛顿流体，计算得到的分子量为 1.48×10 Da。1%（w/v）GH 水溶液的绝对粘度在 NaCl 中从 25 mPa s 降至 10 mPa s，在 CaCl 中降至 7 mPa s，表明 GH 具有聚电解质性质。
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