Ranasinghe Koshila, Gunathilaka Nayana, Amarasinghe Deepika, Udayanga Lahiru
Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Ragama, Sri Lanka.
Department of Parasitology, Faculty of Medicine, University of Kelaniya, Ragama, Sri Lanka.
J Trop Med. 2020 Sep 2;2020:7915035. doi: 10.1155/2020/7915035. eCollection 2020.
Some arbovirus infections, especially dengue, have increased rapidly over the last few decades in Sri Lanka. Prevalence and distribution of different mosquito species have been limitedly documented, which remains grossly inadequate in providing evidence for potential health risks. In this study, the diversity and species composition of mosquitoes in four selected districts in Sri Lanka (Kurunegala, Gampaha, Kegalle, and Kandy) were investigated. Entomological surveys were conducted from a total of 160 temporary and permanent mosquito breeding habitats identified in the study area from June 2017 to October 2018. Mosquito immature stages were sampled using standard dipping, siphoning, or pipetting methods and identified up to the species level. Percentage relative abundance and habitat characteristics such as species richness, dominance, and Shannon-Weiner diversity were calculated for each surveyed habitat type. Associations between co-occurring species were estimated by Hulbert's coefficient of interspecific association (C8). A total of 4663 mosquito larvae belonging to seven genera and fifteen species of mosquitoes were collected. The relative distribution of mosquito species differed significantly among the four studied districts ( = 143.248; = 33; < 0.001). According to Kruskal-Wallis statistics ( < 0.05 at 95% of significance), all diversity indices for immature stages of medically important mosquitoes varied significantly across different breeding sites. Paddy fields had the significantly highest species richness of 4.0 ± 2.82. The coefficients of interspecific association among all the recorded medically important vector mosquitoes were found negative during the present study. The findings of the current study would be useful to identify the entomological potential for disease transmission and facilitate the implementation of appropriate vector control interventions. This would ultimately provide an avenue to improve the personal skills of health staff rather than limiting their knowledge to specified disease vectors, under which the control program is concerned.
在过去几十年里，斯里兰卡一些虫媒病毒感染，尤其是登革热感染迅速增加。不同蚊种的流行情况和分布情况记录有限，这在为潜在健康风险提供证据方面仍严重不足。在本研究中，对斯里兰卡四个选定地区（库鲁内格勒、甘帕哈、凯格勒和康提）的蚊子多样性和物种组成进行了调查。2017年6月至2018年10月，在研究区域共确定了160个临时和永久性蚊子繁殖栖息地，并进行了昆虫学调查。采用标准的 dipping、虹吸或移液方法对蚊子幼虫阶段进行采样，并鉴定到物种水平。计算每个调查栖息地类型的相对丰度百分比和栖息地特征，如物种丰富度、优势度和香农 - 维纳多样性。通过Hulbert种间关联系数（C8）估计共存物种之间的关联。共收集到属于7个属15种蚊子的4663只蚊幼虫。四个研究地区的蚊种相对分布差异显著（χ² = 143.248；自由度 = 33；P < 0.001）。根据Kruskal - Wallis统计（在95%显著性水平下P < 0.05），医学上重要蚊子幼虫阶段的所有多样性指数在不同繁殖地点差异显著。稻田的物种丰富度显著最高，为4.0 ± 2.82。在本研究中，所有记录的医学上重要的病媒蚊子之间的种间关联系数均为负。本研究结果将有助于确定疾病传播的昆虫学潜力，并促进实施适当的病媒控制干预措施。这最终将为提高卫生工作人员的个人技能提供一条途径，而不是将他们的知识局限于控制计划所关注的特定病媒。
