Department of Zoology and Environmental Management, Faculty of Science, University of Kelaniya, Dalugama, Kelaniya, Sri Lanka GQ 11600.
Biomed Res Int. 2020 Apr 28;2020:4602084. doi: 10.1155/2020/4602084. eCollection 2020.
A mosquito species has its own favourable requirements of abiotic and biotic characteristics including microbiota, in a breeding habitat. Some of the microbiota may cause parasitic or pathogenic effects to mosquito larvae such as species of viruses, parasitic bacteria, fungi, protists, entomopathogenic nematodes, and filamentous fungi. In Sri Lanka, there is a scarcity of information on microbiota associated with mosquito breeding habitats and their effect on mosquito larvae. Hence, the present study was conducted to determine microbiota species/taxa associated with a variety of mosquito breeding habitats in selected areas of the Gampaha District in Sri Lanka and the relationship, if any, the microbiota has with mosquito larva survival and breeding. Forty-five microbiota species belonging to 11 phyla were found from different mosquito breeding habitats with the highest percentage belonging to phylum Euglenozoa (27.89%). Species that belonged to the phylum Amoebozoa (1.22%) and Sarcodina (1.17%) had the lowest abundance, and each of its species richness was recorded as one. followed by comprised 30.8% and 16.59%, respectively, of the total rotifer population. From the total microbiota, 25-50% existed as accidental while less than 25% rare, in the habitat type according to their abundance. Paddy fields had the highest species richness (17), evenness (23.52), Shannon-Weiner (66.64), and beta diversity (0.65) over 50% indicating high heterogeneity in microbiota composition among the habitats. Ciliated protists, namely, , spp., and sp., were identified as naturally occurring microbiota associated with mosquito larvae that inhabited in paddy fields and associated irrigation canals. Only caused a significant lethal effect on mosquito larvae. This study revealed that species of , , , , and served as hosts for where infectivity rate in reached 73.22. sp. selectively served as endoparasitic to larvae causing only 4.58% mortality, and invasive cysts of the pathogen were observed in the subcuticular layer of the host body. Even though spp. were found on larvae, there was no lethal effect due to the attachment of the parasitic agent. The potential of these microbiotas in integrated vector controlling approaches in future perspectives is recommended.
一种蚊子物种在其繁殖栖息地中对非生物和生物特征（包括微生物群）有其自身的有利要求。一些微生物群可能对蚊子幼虫产生寄生或致病影响，例如病毒、寄生细菌、真菌、原生动物、昆虫病原线虫和丝状真菌等物种。在斯里兰卡，有关与蚊子繁殖栖息地相关的微生物群及其对蚊子幼虫影响的信息很少。因此，本研究旨在确定与斯里兰卡甘帕哈区选定地区各种蚊子繁殖栖息地相关的微生物物种/分类群，以及微生物群与蚊子幼虫存活和繁殖的任何关系。从不同的蚊子繁殖栖息地中发现了 45 种属于 11 门的微生物物种，其中以 Euglenozoa 门（27.89%）的比例最高。属于 Amoebozoa 门（1.22%）和 Sarcodina 门（1.17%）的物种丰度最低，每种物种的丰富度均记录为 1 种。紧随其后的是，分别占总轮虫种群的 30.8%和 16.59%。在栖息地类型中，根据其丰度，总微生物群中有 25-50%是偶然存在的，而不到 25%是罕见的。稻田的物种丰富度最高（17），均匀度最高（23.52），香农-威纳（66.64）和β多样性（0.65）均超过 50%，表明栖息地之间的微生物群落组成具有高度异质性。纤毛原生动物，即，，，和，被鉴定为与栖息在稻田和相关灌溉渠中的蚊子幼虫自然相关的微生物群。只有引起蚊子幼虫的显著致死作用。本研究表明，，，，和等物种是蚊子幼虫的宿主，其中感染率在达到 73.22%。选择性地作为内寄生宿主，仅导致 4.58%的死亡率，并且在宿主身体的表皮下层观察到病原体的侵袭性囊肿。尽管在蚊子幼虫上发现了 spp.，但由于寄生剂的附着，没有致命作用。建议在未来的视角中，将这些微生物群纳入综合病媒控制方法中。
