Aslani Somayeh, Rahbarghazi Reza, Rahimzadeh Sevda, Rajabi Hadi, Abhari Alireza, Sakhinia Ebrahim
Department of Biochemistry and Clinical Laboratories , School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Mol Cell Med. 2020 Spring;9(2):140-146. doi: 10.22088/IJMCM.BUMS.9.2.140. Epub 2020 Aug 10.
miRNAs are known as the cellular phenomena regulators that exert their effects in post-transcriptional level. Recent studies highlight the role of miRNAs in mesenchymal stem cells differentiation into osteoblasts. The purpose of this study was to recognize the pattern of miRNA-101a-3p and miRNA-200a expression during osteoblastic differentiation of human adipose tissue-derived mesenchymal stem cells. The cells were incubated in osteoblastic differentiation medium for a period of 21 days. Alizarin red S staining was performed to confirm the successful differentiation of adipose-derived mesenchymal stem cells into osteoblast cells. The expression levels of miRNA-101a-3p and miRNA-200a were analyzed by real-time PCR during 0, 7, 14, and 21 days after differentiation induction. Data exhibited the increase of extracellular red color deposition which was evident at the end of the incubation period. The expression of miRNA-101a-3p and miRNA-200a was up regulated during adipose-derived mesenchymal stem cells trans-differentiation into osteoblast-like cells. These miRNAs could be potential novel biomarkers for monitoring successful differentiation of mesenchymal stem cells toward osteoblasts.
微小RNA（miRNAs）是已知的细胞现象调节因子，在转录后水平发挥作用。最近的研究突出了miRNAs在间充质干细胞向成骨细胞分化中的作用。本研究的目的是识别miRNA - 101a - 3p和miRNA - 200a在人脂肪组织来源的间充质干细胞成骨分化过程中的表达模式。将细胞在成骨分化培养基中培养21天。进行茜素红S染色以确认脂肪来源的间充质干细胞成功分化为成骨细胞。在分化诱导后的0、7、14和21天，通过实时聚合酶链反应分析miRNA - 101a - 3p和miRNA - 200a的表达水平。数据显示细胞外红色沉积增加，在培养期结束时很明显。在脂肪来源的间充质干细胞转分化为成骨样细胞的过程中，miRNA - 101a - 3p和miRNA - 200a的表达上调。这些微小RNA可能是监测间充质干细胞向成骨细胞成功分化的潜在新型生物标志物。
