Zhang Y L, Pan W Q
Department of Tropical Diseases, Faculty of Naval Medicine, Naval Medical University, Shanghai 200433, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2020 Jul 27;32(4):331-334. doi: 10.16250/j.32.1374.2020214.
With the rapid development of molecular biology, the isothermal amplification technique has been used for the nucleic acid detection of parasites and other pathogens due to its high efficiency and rapid and simple procedures, and has become an important tool to promote the field detection and control of parasitic diseases. Recombinase-aided isothermal amplification assay (RAA), a novel isothermal amplification technique, which is simple and easy to perform, rapid for field detection, no need for high-end equipment, and rapid field detection, may amplify the target gene fragments within 5 to 20 min under an isothermal condition (usually 37 to 42 ℃) and achieve a real-time observation of the amplification results. RAA has been successfully employed for the nucleic acid detection of a wide range of parasites and other pathogens to date, and has shown a high sensitivity and specificity. Notably, such an assay is suitable for the large-scale field detection in non-lab environments, and is therefore considered to have a potential value of application in rapid field detections.
随着分子生物学的快速发展，等温扩增技术因其高效、操作快速简便，已被用于寄生虫及其他病原体的核酸检测，成为推动寄生虫病现场检测与防控的重要工具。重组酶辅助等温扩增分析（RAA）是一种新型等温扩增技术，操作简单易行，适用于现场快速检测，无需高端设备，可在等温条件下（通常为37至42℃）5至20分钟内扩增目标基因片段，并实现对扩增结果的实时观察。迄今为止，RAA已成功用于多种寄生虫及其他病原体的核酸检测，并显示出高灵敏度和特异性。值得注意的是，该分析方法适用于非实验室环境下的大规模现场检测，因此被认为在现场快速检测中具有潜在的应用价值。
