佛波酯激活的信号通路并不直接参与胰岛素在大鼠脂肪细胞中的作用的证据。

Evidence that phorbol ester-activated pathways are not directly involved in the action of insulin in rat adipocytes.

作者信息

Andersen P H, Richelsen B, Juhl H

机构信息

University Clinic of Endocrinology and Internal Medicine, Aarhus C, Denmark.

出版信息

Biochem Biophys Res Commun. 1988 Jul 15;154(1):171-8. doi: 10.1016/0006-291x(88)90666-3.

DOI:10.1016/0006-291x(88)90666-3

PMID:3293563

Abstract

The effects of 12-phorbol 13-myristate acetate (PMA) on glucose transport, glucose metabolism and lipolysis in rat adipocytes were examined. Alone, PMA (100 ng/ml) stimulated 2-deoxyglucose transport, glucose oxidation and lipogenesis by an amount corresponding to about 30-50% of the maximal insulin effect. The effect of PMA on the insulin-stimulated processes was additive at all insulin concentrations. Adenosine deaminase-stimulated lipolysis was enhanced by about 50% by PMA (100 ng/ml). Thus, in contrast to insulin, PMA had a lipolytic effect. The antilipolytic effect of insulin was unaffected by the presence of PMA, both ED50 and maximal inhibition of insulin were unchanged. In conclusion, it is suggested that phorbol esters may activate pathways other than those activated by insulin in rat adipocytes.

摘要

研究了12 - 佛波醇13 - 肉豆蔻酸酯乙酸酯（PMA）对大鼠脂肪细胞葡萄糖转运、葡萄糖代谢和脂肪分解的影响。单独使用时，PMA（100 ng/ml）刺激2 - 脱氧葡萄糖转运、葡萄糖氧化和脂肪生成，其作用程度相当于最大胰岛素效应的约30 - 50%。在所有胰岛素浓度下，PMA对胰岛素刺激过程的作用都是相加的。PMA（100 ng/ml）使腺苷脱氨酶刺激的脂肪分解增强约50%。因此，与胰岛素相反，PMA具有脂肪分解作用。胰岛素的抗脂肪分解作用不受PMA存在的影响，胰岛素的半数有效剂量（ED50）和最大抑制作用均未改变。总之，提示佛波酯可能激活大鼠脂肪细胞中除胰岛素激活的途径以外的其他途径。