L-茶氨酸通过膳食纤维喂养调节短链脂肪酸代谢影响肠道黏膜免疫。
L-Theanine affects intestinal mucosal immunity by regulating short-chain fatty acid metabolism under dietary fiber feeding.
机构信息
Key Lab of Tea Science of Ministry of Education, Hunan Agricultural University, Changsha, Hunan 410128, China and National Research Center of Engineering Technology for Utilization of Botanical Functional Ingredients, Hunan Agricultural University, Changsha, Hunan 410128, China and Hunan Agricultural University, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients, Changsha, Hunan 410128, China.
出版信息
Food Funct. 2020 Sep 23;11(9):8369-8379. doi: 10.1039/d0fo01069c.
To investigate the effects of l-Theanine (LTA) on intestinal mucosal immunity and the regulation of short-chain fatty acid (SCFA) metabolism under dietary fiber feeding, a 28-day feeding experiment was performed in Sprague-Dawley rats. The results show that LTA increased the proportion of Prevotella, Lachnospira, and Ruminococcus while increasing the total SCFA, acetic acid, propionic acid, and butyric acid contents in the feces. LTA also increased IgA, IgE, and IgG levels in the ileum, and increased villi height and crypt depth. Moreover, LTA upregulated the mRNA and protein expression of acetyl-CoA carboxylase 1, sterol element-binding protein 1c, fatty acid synthase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver, while downregulating the expression of glucose-6-phosphatase and phosphoenolpyruvate carboxykinase 1 in the colon. Our study suggests that LTA can affect intestinal mucosal immunity by regulating SCFA metabolism under dietary fiber feeding.
为了研究茶氨酸（LTA）在膳食纤维喂养下对肠道黏膜免疫和短链脂肪酸（SCFA）代谢的调节作用，我们对 Sprague-Dawley 大鼠进行了为期 28 天的喂养实验。结果表明，LTA 增加了普雷沃氏菌属、毛螺菌属和瘤胃球菌属的比例，同时增加了粪便中总 SCFA、乙酸、丙酸和丁酸的含量。LTA 还增加了回肠中的 IgA、IgE 和 IgG 水平，并增加了绒毛高度和隐窝深度。此外，LTA 上调了肝脏中乙酰辅酶 A 羧化酶 1、固醇元件结合蛋白 1c、脂肪酸合成酶和 3-羟-3-甲基戊二酰辅酶 A 还原酶的 mRNA 和蛋白表达，同时下调了结肠中葡萄糖-6-磷酸酶和磷酸烯醇丙酮酸羧激酶 1 的表达。我们的研究表明，LTA 可以通过调节膳食纤维喂养下的 SCFA 代谢来影响肠道黏膜免疫。
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