Schlimok G, Funke I, Bock B, Schweiberer B, Riethmüller G
Institut für Immunologie, Universität München.
Arzneimittelforschung. 1988 Mar;38(3A):435-7.
Monoclonal antibody CK2, recognizing component No. 18, appeared to be the most suitable reagent for the detection of epithelial tumor cells in bone marrow. Its specificity was confirmed in a double-marker staining procedure (combination of APAAP-technique and radioautography). CK2 positive cells were demonstrated not to reveal any cross-reactivity with an antibody directed against the "leucocyte common antigen". A significant correlation between the presence of epithelial tumor cells in bone marrow and certain conventional risk factors was found. A more detailed phenotypic characterisation could demonstrate the expression of proliferation associated antigens on these cells. Furthermore in an immunotherapeutic approach with monoclonal antibody 17-1A, labelling of the disseminated tumor cells in bone marrow after infusion of the antibody was shown.
识别第18号成分的单克隆抗体CK2似乎是检测骨髓中上皮肿瘤细胞最合适的试剂。其特异性在双标记染色程序（碱性磷酸酶抗碱性磷酸酶技术与放射自显影相结合）中得到证实。已证明CK2阳性细胞与针对“白细胞共同抗原”的抗体无任何交叉反应。发现骨髓中上皮肿瘤细胞的存在与某些传统危险因素之间存在显著相关性。更详细的表型特征可以证明这些细胞上增殖相关抗原的表达。此外，在使用单克隆抗体17-1A的免疫治疗方法中，显示了抗体注入后骨髓中播散肿瘤细胞的标记情况。
