Güçlü Ertuğrul, Koroglu Mehmet, Yürümez Yusuf, Toptan Hande, Kose Elif, Güneysu Fatih, Karabay Oğuz
. Sakarya University Faculty of Medicine, Department of Infectious Diseases and Clinical Microbiology, Sakarya, Turkey.
. Sakarya University Faculty of Medicine, Department of Clinic Microbiology, Sakarya, Turkey.
Rev Assoc Med Bras (1992). 2020 Aug;66(8):1116-1121. doi: 10.1590/1806-9282.66.8.1116.
Healthcare personnel are at risk of becoming infected while taking upper and/or lower respiratory tract specimens. Therefore, there is a need for sampling methods that do not risk infecting them. In this study, we aimed to compare the saliva and Oro-Nasopharyngeal Swab (ONS) sampling methods.
Patients were divided into three groups. Group 1 included patients whose diagnosis of COVID-19 was confirmed by polymerase chain reaction (PCR). Group 2 included patients with COVID-19 compatible findings in lung computed tomography (CT), but with a negative PCR. Group 3 included patients who presented to the emergency department with COVID-19 compatible complaints but had normal CT. Saliva and ONS samples were taken on the third day of hospitalization in groups 1 and 2, whereas in group 3, they were taken at the time of admission to the hospital.
A total of 64 patients were included in the study. The average age was 51.04 ± 17.9 years, and 37 (57.8%) were male. SARS-CoV-2 was detected in 27 (42.2%) patients' saliva samples. While the sensitivity and positive predictive value of saliva samples were 85.2%, specificity and negative predictive value were 89.2%. The value of kappa was in substantial agreement (0.744), and it was found statistically significant (<0.001).
Saliva samples can be used instead of ONS samples in detecting SARS-CoV-2. Investigating SARS-CoV-2 with saliva is cheaper, easier for the patient and overall, and, most importantly, it poses much less risk of SARS-CoV-2 contamination to healthcare personnel.
医护人员在采集上呼吸道和/或下呼吸道标本时存在感染风险。因此，需要采用不会对他们造成感染风险的采样方法。在本研究中，我们旨在比较唾液和口鼻咽拭子（ONS）采样方法。
将患者分为三组。第1组包括经聚合酶链反应（PCR）确诊为COVID-19的患者。第2组包括肺部计算机断层扫描（CT）有COVID-19相关表现但PCR检测为阴性的患者。第3组包括因COVID-19相关症状就诊于急诊科但CT正常的患者。第1组和第2组在住院第三天采集唾液和ONS样本，而第3组在入院时采集。
本研究共纳入64例患者。平均年龄为51.04±17.9岁，男性37例（57.8%）。27例（42.2%）患者的唾液样本中检测到SARS-CoV-2。唾液样本的敏感性和阳性预测值为85.2%，特异性和阴性预测值为89.2%。kappa值具有高度一致性（0.744），且具有统计学意义（<0.001）。
在检测SARS-CoV-2时，唾液样本可替代ONS样本。用唾液检测SARS-CoV-2成本更低，对患者来说更简便，总体而言，最重要的是，它对医护人员造成SARS-CoV-2污染的风险要小得多。
