An electrophysiological laminar analysis of single somatosensory neurons in partially deafferented rat hindlimb granular cortex subsequent to transection of the sciatic nerve.

A sample of 302 neurons in rat hindlimb granular cortex was studied between 2 and 3 weeks after transection and ligation of the sciatic nerve. These neurons were compared to a control sample obtained from normal rats under similar experimental conditions. After sciatic nerve transection the proportion of neurons driven by somatic stimuli applied to the hindlimb (29.1%) was not significantly different from the proportion observed in the control sample (25.8%). The proportion of neurons with cutaneous receptive fields was also the same before and after nerve transection although the proportion of neurons responding to higher threshold taps was reduced. Spontaneously active neurons were encountered more frequently after sciatic nerve transection than in the control (45% vs 32%) and their mean discharge frequency was higher (8.6 vs 6.4 imp/s). Twice as many (10.2% vs 5.2%) spontaneously active neurons whose discharge was modulated by afferent stimuli were found after sciatic nerve transection. This and several other lines of evidence suggest that the cortical neurons were released from inhibition by the sciatic nerve transection. The number of spontaneously active neurons and the mean discharge rate were increased in each cortical lamina suggesting that the increased excitability seen after deafferentation occurred in all cortical layers. Neurons throughout the portion of the hindlimb representation studied could be driven from the remaining innervated region of the hindlimb, including one and sometimes two digits, part of the palm and most of the dorsum of the foot. More neurons were driven by receptive fields on the ankle than was the case for normal rats. Most neurons with cutaneous receptive fields were distributed in the same layers as those in the control group and had normal shapes and appearances. However, there were few of small size. Most were of moderate dimensions well within the normal range. Seven examples were found with unusually large proportions of their field extending from the foot onto the ankle and covering most of the posterior quadrant of the animal. In normal cortex the largest receptive fields were found in the middle layers. This distribution was not as clear after sciatic nerve section and a mixture of large and medium-sized receptive fields occurred at each depth. Further, some neurons with cutaneous receptive fields were found above 300 micron and below 1000 micron, depths where receptive fields were uncommon in the normal sample.