Clark R, Stampfer M R, Milley R, O'Rourke E, Walen K H, Kriegler M, Kopplin J, McCormick F
Cetus Corporation, Emeryville, California 94608.
Cancer Res. 1988 Aug 15;48(16):4689-94.
We have introduced viral oncogenes into human mammary epithelial cells through the use of murine retroviruses. A continuous cell line (184A1N4) derived from benzo(a)pyrene treatment of normal breast epithelial cells was used as a recipient for the ras, mos, and T-antigen oncogenes. Each of these oncogenes enabled the 184A1N4 cells to grow in a selective medium, thus demonstrating the potential utility of these cells for oncogene detection and isolation. 184A1N4 cells transformed by T-antigen were nontumorigenic in athymic mice, but v-ras transformants were weakly tumorigenic. Transformants bearing both the T-antigen and ras oncogenes were strongly tumorigenic, however. The karyotype of these double transformants shows a high degree of stability. These results demonstrate the stepwise acquisition of the fully malignant phenotype by normal human epithelial cells in vitro.
我们通过使用鼠逆转录病毒将病毒癌基因导入人乳腺上皮细胞。用苯并(a)芘处理正常乳腺上皮细胞得到的连续细胞系(184A1N4)被用作ras、mos和T抗原癌基因的受体。这些癌基因中的每一个都能使184A1N4细胞在选择性培养基中生长，从而证明了这些细胞在癌基因检测和分离方面的潜在用途。经T抗原转化的184A1N4细胞在无胸腺小鼠中无致瘤性，但v-ras转化体有弱致瘤性。然而，携带T抗原和ras癌基因的转化体具有强致瘤性。这些双转化体的核型显示出高度的稳定性。这些结果证明了正常人上皮细胞在体外逐步获得完全恶性表型的过程。
