School of Life Science, South China Normal University, Guangzhou, China.
Guangdong Provincial Engineering Technology Research Center for Drug and Food Biological Resources Processing and Comprehensive Utilization, Guangzhou, China.
Lett Appl Microbiol. 2021 Feb;72(2):149-156. doi: 10.1111/lam.13387. Epub 2020 Nov 8.
Spore-forming Bacillus sp. has been extensively studied for their probiotic properties. In this study, an acid-treated rice straw hydrolysate was used as carbon source to produce the spores of Bacillus coagulans. The results showed that this hydrolysate significantly improved the spore yield compared with other carbon sources such as glucose. Three significant medium components including rice straw hydrolysate, MnSO and yeast extract were screened by Plackett-Burman design. These significant variables were further optimized by response surface methodology (RSM). The optimal values of the medium components were rice straw hydolysate of 27% (v/v), MnSO of 0·78 g l and yeast extract of 1·2 g l . The optimized medium and RSM model for spore production were validated in a 5 l bioreactor. Overall, this sporulation medium containing acid-treated rice straw hydrolysate has a potential to be used in the production of B. coagulans spores.
产芽孢杆菌已被广泛研究其益生菌特性。在这项研究中，酸处理的稻草水解物被用作碳源来生产凝结芽孢杆菌的孢子。结果表明，与葡萄糖等其他碳源相比，该水解物显著提高了芽孢的产量。通过 Plackett-Burman 设计筛选出稻草水解物、MnSO 和酵母提取物这三种显著的培养基成分。通过响应面法（RSM）进一步优化这些显著变量。培养基成分的最佳值为 27%（v/v）稻草水解物、0.78 g l 的 MnSO 和 1.2 g l 的酵母提取物。优化后的培养基和用于孢子生产的 RSM 模型在 5 L 生物反应器中进行了验证。总的来说，这种含有酸处理稻草水解物的孢子形成培养基有可能用于生产凝结芽孢杆菌的孢子。
