Hill J A, Faris H M, Schiff I, Anderson D J
Fearing Research Laboratory, Department of Obstetrics, Gynecology, and Reproductive Biology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Fertil Steril. 1988 Aug;50(2):216-22.
Monoclonal antibodies identifying leukocytes subpopulations were applied to smears of laparoscopically collected peritoneal fluid leukocytes and parallel samples of peripheral blood leukocytes from women with endometriosis (n = 33), those with unexplained infertility (n = 9), and fertile controls (n = 8). Peripheral blood leukocyte profiles in all groups were indistinguishable from reported normal values. Peritoneal fluid leukocyte profiles were observed to be different between groups. The most significant elevations in total leukocytes, macrophages, helper T lymphocytes and natural-killer cells were observed in women with stage I and II endometriosis. Significantly elevated levels of total leukocytes, macrophages, and T lymphocytes were also observed in peritoneal fluid from women with unexplained infertility. The results from this study indicate that the peritoneal environment is immunologically dynamic and suggest that cellular immune mechanisms may contribute to reproductive failure in women with endometriosis and unexplained infertility.
将识别白细胞亚群的单克隆抗体应用于腹腔镜采集的腹腔液白细胞涂片以及来自患有子宫内膜异位症的女性（n = 33）、不明原因不孕症女性（n = 9）和可育对照女性（n = 8）的外周血白细胞平行样本。所有组的外周血白细胞谱与报道的正常值无差异。观察到各组间腹腔液白细胞谱不同。在I期和II期子宫内膜异位症女性中观察到总白细胞、巨噬细胞、辅助性T淋巴细胞和自然杀伤细胞的升高最为显著。在不明原因不孕症女性的腹腔液中也观察到总白细胞、巨噬细胞和T淋巴细胞水平显著升高。本研究结果表明腹腔环境具有免疫动态性，并提示细胞免疫机制可能导致患有子宫内膜异位症和不明原因不孕症的女性出现生殖失败。
