1Research and Development Station for Bovine Arad, Arad, 310059, Romania; 2Research and Development Institute for Bovine Balotești, Balotești, 077015, Romania.
China Agricultural University, College of Animal Science and Technology, Beijing, 100193, China.
Acta Biochim Pol. 2020 Sep 17;67(3):341-346. doi: 10.18388/abp.2020_5080.
Simmental and Holstein cattle, being among the most widely distributed breeds worldwide, have been subjected to continuous selection for distinct purposes. In the current study, we evaluated the levels of SNPs identified through the use of SNP assay in Romanian Holstein and Romanian Simmental cattle, which then were compared to the data from Chinese Holstein and Chinese Simmental cattle. In total, 282 animals were genotyped: Romanian Holstein (n=30), Romanian Simmental (n=22), Chinese Holstein (n=96) and Chinese Simmental cattle (n=136), using 39,724 common SNPs to analyze minor allele frequency, genetic variability and level of SNPs. Among studied breeds, the average percentage of polymorphic markers was 90.84%, with the highest value in Chinese Simmental (91.37%) and lowest in Romanian Simmental cattle (90.31%). The average HO ranged from 0.426 in Romanian Holstein to 0.416 in Romanian Simmental, and from 0.425 in Chinese Holstein to 0.422 in Chinese Simmental. The distribution of SNPs was homogenous across the breeds, except the Romanian Simmental which displayed the lowest percentage of polymorphic markers (24,66 and 32,48%) from higher MAF category (0.3 to <0.4 and 0.4 to <0.5) and the highest percentage (3.82 and 12.00%) for SNPs from low and intermediate MAF categories (0.05 to <0.1 and 0.1 to <0.2). In the current study, the SNP assay was successfully used to analyze the level of SNP sites of Romanian cattle breeds, however, a higher number of samples and production data are needed for future applications of the results in genomic selection, genome-wide association studies and genetic diversity analysis.
西门塔尔牛和荷斯坦牛是世界上分布最广的品种之一，它们一直以来都在为不同的目的进行持续的选择。在本研究中，我们评估了通过 SNP 检测在罗马尼亚荷斯坦牛和罗马尼亚西门塔尔牛中鉴定的 SNP 水平，并将其与中国荷斯坦牛和中国西门塔尔牛的数据进行比较。总共有 282 头动物进行了基因分型：罗马尼亚荷斯坦牛（n=30）、罗马尼亚西门塔尔牛（n=22）、中国荷斯坦牛（n=96）和中国西门塔尔牛（n=136），使用 39724 个常见 SNP 来分析次要等位基因频率、遗传变异和 SNP 水平。在所研究的品种中，多态性标记的平均百分比为 90.84%，其中中国西门塔尔牛最高（91.37%），罗马尼亚西门塔尔牛最低（90.31%）。平均 HO 范围从罗马尼亚荷斯坦牛的 0.426 到罗马尼亚西门塔尔牛的 0.416，从中国荷斯坦牛的 0.425 到中国西门塔尔牛的 0.422。除罗马尼亚西门塔尔牛外，SNP 的分布在各品种之间是均匀的，罗马尼亚西门塔尔牛的高 MAF 类别（0.3 至<0.4 和 0.4 至<0.5）的多态性标记百分比最低（24、66 和 32.48%），低和中 MAF 类别（0.05 至<0.1 和 0.1 至<0.2）的 SNP 百分比最高（3.82 和 12.00%）。在本研究中，SNP 检测成功用于分析罗马尼亚牛品种的 SNP 位点水平，但为了在基因组选择、全基因组关联研究和遗传多样性分析中应用结果，需要更多的样本和生产数据。
