Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Ceará, Brazil.
Laboratório de Bioquímica de Plantas laticíferas (LABPL), Universidade Federal do Delta do Parnaíba, Parnaíba, Piauí, Brazil.
Prep Biochem Biotechnol. 2021;51(4):375-385. doi: 10.1080/10826068.2020.1818258. Epub 2020 Sep 17.
produces a milky sap containing proteolytic enzymes. At low concentrations, they induce milk-clotting (60 µg/ml) and to dehair hides (0.05 and 0.1%). A protocol for obtaining the enzymes is reported. The latex was mixed with distilled water and the mixture was cleaned through centrifugation. It was dialyzed with distilled water and centrifuged again to recover the soluble fraction [EP]. The dialyze is a key feature of the process. EP was characterized in terms of protein profile, chemical stability, among other criteria. Wild plants belonging to ten geographic regions and grown in different ecological conditions were used as latex source. Collections were carried out, spaced at three-month, according to the seasons at the site of the study. Proteolytic activity was measured as an internal marker and for determining stability of the samples. EP was also analyzed for metal content and microbiology. EP showed similar magnitude of proteolysis, chromatographic and electrophoretic profiles of proteins. Samples stored at 25 °C exhibited reduced solubility (11%) and proteolytic capacity (11%) after six months. Enzyme autolysis was negligible. Microbiological and metal analyses revealed standard quality of all the samples tested. EP induced milk clotting and hide dehairing after storage for up to six months.
产生产生乳白色汁液，其中含有蛋白水解酶。在低浓度下，它们能诱导牛奶凝结（60μg/ml）并脱毛（0.05 和 0.1%）。本文报道了一种获取酶的方法。乳胶与蒸馏水混合，混合物通过离心进行净化。用蒸馏水透析并再次离心以回收可溶部分[EP]。透析是该过程的一个关键特征。根据蛋白图谱、化学稳定性等标准对 EP 进行了表征。从属于十个地理区域的野生植物被用作乳胶的来源，这些植物在不同的生态条件下生长。根据研究地点的季节，每三个月进行一次收集。以蛋白酶活性作为内部标记来测量，并确定样品的稳定性。还对 EP 进行了金属含量和微生物学分析。EP 显示出相似的蛋白水解、色谱和电泳图谱。在六个月后，储存在 25°C 下的样品的溶解度（11%）和蛋白水解能力（11%）降低。酶自溶可以忽略不计。微生物学和金属分析显示，所有测试样品的质量均符合标准。EP 在储存长达六个月后仍能诱导牛奶凝结和脱毛。
