May G S, Morris N R
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Dev Biol. 1988 Aug;128(2):406-14. doi: 10.1016/0012-1606(88)90302-8.
A beta-tubulin gene previously suggested to participate in conidial development in the filamentous fungus Aspergillus nidulans is shown to be developmentally regulated in its expression. A quantitative S1 assay was used to show that the abundance of the tubC messenger RNA increases during conidial development relative to the benA messenger RNA. Morphological analysis of cultures, used to prepare RNA for the S1 analysis, demonstrated that the increase in tubC messenger RNA was directly correlated with the appearance of conidiating cell types and structures and that as these cell types become more prevalent in the culture, the level of the messenger RNA increases. Two-dimensional gel electrophoresis of labeled proteins showed that the relative increase in tubC messenger RNA was reflected in an increase in the tubC protein beta 3. RNA blot hybridization analysis and in vitro translation of total RNA from mycelia and conidia were used to demonstrate that both benA and tubC beta-tubulin messenger RNAs are absent from conidia.
先前有研究表明，在丝状真菌构巢曲霉中参与分生孢子发育的一个β-微管蛋白基因，其表达受发育调控。采用定量S1分析方法显示，相对于benA信使核糖核酸，tubC信使核糖核酸的丰度在分生孢子发育过程中增加。用于为S1分析制备RNA的培养物形态学分析表明，tubC信使核糖核酸的增加与产孢细胞类型和结构的出现直接相关，并且随着这些细胞类型在培养物中变得更加普遍，信使核糖核酸的水平也会增加。标记蛋白质的二维凝胶电泳显示，tubC信使核糖核酸的相对增加反映在tubC蛋白β3的增加上。利用RNA印迹杂交分析以及菌丝体和分生孢子总RNA的体外翻译，证明分生孢子中不存在benA和tubCβ-微管蛋白信使核糖核酸。
