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编码链球菌免疫球蛋白A蛋白酶的基因克隆及其在大肠杆菌中的表达。

Cloning of the gene encoding streptococcal immunoglobulin A protease and its expression in Escherichia coli.

作者信息

Gilbert J V, Plaut A G, Fishman Y, Wright A

机构信息

Department of Medicine, Tufts-New England Medical Center Hospital, Boston, Massachusetts.

出版信息

Infect Immun. 1988 Aug;56(8):1961-6. doi: 10.1128/iai.56.8.1961-1966.1988.

DOI:10.1128/iai.56.8.1961-1966.1988
PMID:3294181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259508/
Abstract

We have identified and cloned a 6-kilobase-pair segment of chromosomal DNA from Streptococcus sanguis ATCC 10556 that encodes immunoglobulin A (IgA) protease activity when cloned into Escherichia coli. The enzyme specified by the iga gene in plasmid pJG1 accumulates in the periplasm of E. coli MM294 cells and has a substrate specificity for human IgA1 identical to that of native S. sanguis protease. Hybridization experiments with probes from within the encoding DNA showed no detectable homology at the nucleotide sequence level with chromosomal DNA of gram-negative bacteria that excrete IgA protease. Moreover, the S. sanguis iga gene probes showed no detectable hybridization with chromosomal DNA of S. pneumoniae, although the IgA proteases of these two streptococcal species cleaved the identical peptide bond in the human IgA1 heavy-chain hinge region.

摘要

我们已从血链球菌ATCC 10556中鉴定并克隆出一段6千碱基对的染色体DNA片段，该片段克隆到大肠杆菌中时可编码免疫球蛋白A（IgA）蛋白酶活性。质粒pJG1中iga基因所指定的酶积聚在大肠杆菌MM294细胞的周质中，对人IgA1的底物特异性与天然血链球菌蛋白酶相同。用编码DNA内的探针进行的杂交实验表明，在核苷酸序列水平上，与分泌IgA蛋白酶的革兰氏阴性菌的染色体DNA没有可检测到的同源性。此外，血链球菌iga基因探针与肺炎链球菌的染色体DNA没有可检测到的杂交，尽管这两种链球菌的IgA蛋白酶在人IgA1重链铰链区切割相同的肽键。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/5de6a5922275/iai00080-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/86fb578d44ae/iai00080-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/e9d7f9d564b8/iai00080-0146-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/5de6a5922275/iai00080-0147-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/86fb578d44ae/iai00080-0146-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/e9d7f9d564b8/iai00080-0146-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cdf/259508/5de6a5922275/iai00080-0147-a.jpg

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本文引用的文献

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Specific proteolysis of human IgA by Streptococcus pneumoniae and Haemophilus influenzae.肺炎链球菌和流感嗜血杆菌对人IgA的特异性蛋白水解作用。
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Bacterial extracellular zinc-containing metalloproteases.细菌细胞外含锌金属蛋白酶
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