State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling 712100, China.
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
Genes (Basel). 2020 Sep 15;11(9):1078. doi: 10.3390/genes11091078.
Alternative splicing (AS) is an important mechanism by which eukaryotes regulate transcription and protein diversity. The dynamic changes in AS that occur on a genome-wide scale during interactions between plant roots and pathogens remain unknown. Here, we used the interaction between Arabidopsis and as a model to explore the AS changes that take place during the response of roots to infection by means of high-throughput RNA-sequencing. We showed that dynamic changes in AS occur much earlier than changes at the level of transcription during infection. Comparing genes that are regulated at the transcriptional and AS levels indicated that there are few common genes between differentially spliced genes (DSGs) and differentially expressed genes (DEGs). The functional gene ontology (GO) analysis identified that the enriched GO terms for the DSGs were different from those of the DEGs. The DSGs were over-represented in GO terms associated with post-transcriptional and translational regulations, suggesting that AS may act on RNA stability and during post-translation, thus affecting the output of plant defense molecules. Meanwhile, changes in DSGs were infection stage-specific. Furthermore, the nucleotide binding domain and leucine-rich repeat proteins and receptor-like kinases, key regulators in plant immunity, were shown to undergo dynamic changes in AS in response to . Taken together, AS, along with transcription, modulates plant root defense to through transcriptome reprogramming.
可变剪接 (AS) 是真核生物调控转录和蛋白质多样性的重要机制。植物根系与病原体相互作用时，基因组范围内 AS 的动态变化尚不清楚。在这里，我们以拟南芥和 的相互作用为模型，通过高通量 RNA 测序来探索根系对感染反应过程中发生的 AS 变化。结果表明，在 感染过程中，AS 的动态变化比转录水平的变化出现得更早。比较转录和 AS 水平调节的基因表明，在差异剪接基因 (DSG) 和差异表达基因 (DEG) 之间很少有共同基因。功能基因本体 (GO) 分析表明，DSG 的富集 GO 术语与 DEG 的富集 GO 术语不同。DSG 在与转录后和翻译后调控相关的 GO 术语中过表达，表明 AS 可能作用于 RNA 稳定性和翻译后过程，从而影响植物防御分子的输出。同时，DSG 的变化具有感染阶段特异性。此外，核苷酸结合域和富含亮氨酸重复蛋白和类受体激酶等植物免疫中的关键调节剂，在响应 时表现出 AS 的动态变化。总之，AS 与转录一起，通过转录组重编程调节植物根系对 的防御。
