Boschi Federico, Schvartzman Claudia, Murchio Sara, Ferreira Virginia, Siri Maria I, Galván Guillermo A, Smoker Matthew, Stransfeld Lena, Zipfel Cyril, Vilaró Francisco L, Dalla-Rizza Marco
Instituto Nacional de SemillasCanelones, Uruguay.
Unidad de Biotecnología, Instituto Nacional de Investigación AgropecuariaCanelones, Uruguay.
Front Plant Sci. 2017 Sep 25;8:1642. doi: 10.3389/fpls.2017.01642. eCollection 2017.
Bacterial wilt (BW) caused by is responsible for substantial losses in cultivated potato () crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to . Here, we evaluated whether the transgenic expression of would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Resistance to was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing showed greater resistance to , with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.
由[病原菌名称未给出]引起的青枯病在全球范围内导致栽培马铃薯（[马铃薯品种未给出]）作物遭受重大损失。已在野生种中鉴定出抗性基因；然而，通过传统育种引入这些基因仅实现了部分抗性，这与不良的农艺性状有关。拟南芥（At）模式识别受体延伸因子-Tu（EF-Tu）受体（EFR）识别细菌病原体相关分子模式EF-Tu（及其衍生肽elf18）以赋予抗菌免疫。先前的研究表明，将AtEFR转入番茄可增强对[病原菌名称未给出]的抗性。在此，我们评估了[AtEFR基因]的转基因表达是否同样会增加商业马铃薯品系（INIA Iporá）以及一个育种马铃薯品系（09509.6）对青枯病的抗性，在09509.6中已从野生马铃薯[野生种名称未给出]导入了数量抗性。通过在可控条件下损伤根系接种来评估对[病原菌名称未给出]的抗性。表达[AtEFR基因]的INIA Iporá和09509.6马铃薯品系均表现出对[病原菌名称未给出]更强的抗性，通过多重PCR和平板计数评估，块茎中未检测到细菌。值得注意的是，AtEFR的表达与从[野生种名称未给出]导入的数量抗性在09509.6-AtEFR品系中具有显著的累加效应。这些结果表明，[AtEFR基因]的异源表达与从野生近缘种导入的数量抗性相结合是在马铃薯中培育抗青枯病能力的一种有前景的策略。
