Abdulkadir Yuksel State Hospital, Gaziantep, Turkey.
Hacettepe University, Ankara, Turkey; and.
Cornea. 2020 Dec;39(12):1533-1540. doi: 10.1097/ICO.0000000000002494.
To evaluate the effects of the application of iontophoresis-assisted rose bengal and green light cross-linking (I-RGX) therapy on enucleated rabbit eyes for corneal biomechanical parameters, dye diffusion rates, and green light levels reaching deep tissues and to compare these parameters with a standard rose bengal and green light cross-linking (RGX) therapy.
Forty-five enucleated rabbit eyes were used in this study. To evaluate biomechanical changes, corneas were divided into the following 4 groups: the control group, the 0.1% rose bengal application group, the RGX group (100 J/cm), and the I-RGX group (100 J/cm). After this, corneal strips were evaluated with a uniaxial extensometer. To assess corneal dye diffusion, postprocedure dye depth was recorded with anterior segment optic coherence tomography. The amount of irradiation passing through the cornea during irradiation with 250 mW/cm irradiation power was measured with a laser power meter at the first, third, and seventh minutes.
In the I-RGX-treated group especially, the mean elastic modulus and corneal stiffness values were about 4.7 times higher when compared with the controls and about 2.2 times higher than those in the RGX group. The rose bengal diffusion depth was 26.63% ± 3.84% of the total corneal thickness in the rose bengal drop group, but this value increased to 42.22% ± 4.77% in the iontophoresis group (<0.001). After iontophoresis, an average of 98% of the 100 J/cm green light was kept in the cornea.
I-RGX is a very useful method for increasing corneal biomechanical strength and is highly effective in increasing the amount of corneal dye diffusion into the cornea while also minimalizing the amount of laser passage reaching deeper tissues.
评估离子导入辅助孟加拉玫瑰红和绿光交联（I-RGX）治疗对去眼兔眼角膜生物力学参数、染料扩散率以及绿光到达深层组织的影响，并将这些参数与标准孟加拉玫瑰红和绿光交联（RGX）治疗进行比较。
本研究使用 45 只去眼兔眼。为了评估生物力学变化，将角膜分为以下 4 组：对照组、0.1%孟加拉玫瑰红组、RGX 组（100 J/cm）和 I-RGX 组（100 J/cm）。然后，使用单轴拉伸计评估角膜条。为了评估角膜染料扩散，用眼前节光学相干断层扫描记录术后染料深度。用激光功率计在第 1、3 和 7 分钟测量在 250 mW/cm 辐照功率下照射时穿过角膜的辐照度。
特别是在 I-RGX 治疗组中，与对照组相比，平均弹性模量和角膜硬度值分别约高 4.7 倍，比 RGX 组高 2.2 倍。在孟加拉玫瑰红滴注组中，孟加拉玫瑰红的扩散深度为角膜总厚度的 26.63%±3.84%，但在离子导入组中，这一数值增加到 42.22%±4.77%（<0.001）。离子导入后，平均 98%的 100 J/cm 绿光保留在角膜中。
I-RGX 是一种非常有用的方法，可以提高角膜生物力学强度，在增加角膜染料扩散到角膜中的量方面非常有效，同时最大限度地减少到达深层组织的激光量。
