Department of Orthopaedic Surgery, Associated Hospital, Beihua University, Jilin, 132013, Jilin, People's Republic of China.
Department of Clinical Diagnosis, School of Laboratory Medicine, Beihua University, No.3999, East Road of Binjiang, Jilin, 132013, Jilin, People's Republic of China.
J Orthop Surg Res. 2020 Sep 18;15(1):428. doi: 10.1186/s13018-020-01954-6.
The diagnosis, treatment, and efficacy evaluation of anterior cruciate ligament (ACL) partial rupture remains controversial. This research aims to investigate the underlying mechanism of partial ACL injuries to the meniscus degeneration in the rabbit knee.
Sixty New Zealand white rabbits were randomly divided into three groups including an experimental group, a sham group (n = 6), and a blank control group (n = 6). The experimental group is composed of an anteromedial bundle (AMB) rupture group (n = 24) and a posterolateral bundle (PLB) rupture group (n = 24). Rabbits in the experimental group were subjected to right hind limbs knee surgery to induce ACL part injury under the arthroscopy. Finally, eight rabbits including 6 in the model group and 2 in the control group were sampled randomly on the 2nd, 4th, and 8th weeks respectively. We observed the typical form of the meniscus through HE staining. Expressions of inflammatory factors including interleukin-1β (IL-1β) and IL-17 in the knee joint fluid were determined by means of an ELISA. Analysis of the mRNA expressions of matrix metalloproteinases-13(MMP-13) was performed to evaluate the inflammatory mediators in the pathogenesis of the meniscus.
HE staining results showed that the surface was rough and the tissues were loose displaying collagen fibers of varying thickness. Both IL-1β and IL-17 in the synovial fluid and the positive rate of MMP-13 in addition to MMP-13 mRNA showed a demonstrable increase treads from the 2nd to the 8th week. The significant difference was found (P < 0.05) compared to the control group.
We conclude that the elevated levels of IL-1β and IL-17, along with increased MMP13 expression, resulted in meniscus degradation in the rabbit knee joint model with partial ACL injury.
前交叉韧带（ACL）部分撕裂的诊断、治疗和疗效评估仍存在争议。本研究旨在探讨兔膝关节 ACL 部分损伤导致半月板退变的潜在机制。
60 只新西兰大白兔随机分为三组，实验组、假手术组（n = 6）和空白对照组（n = 6）。实验组由前内侧束（AMB）破裂组（n = 24）和后外侧束（PLB）破裂组（n = 24）组成。实验组的兔子在关节镜下接受右后肢膝关节手术，以诱导 ACL 部分损伤。最后，第 2、4、8 周分别随机抽取模型组的 6 只和对照组的 2 只兔子进行实验。我们通过 HE 染色观察半月板的典型形态。采用 ELISA 法检测膝关节液中炎症因子白细胞介素-1β（IL-1β）和 IL-17 的表达。分析基质金属蛋白酶-13（MMP-13）的 mRNA 表达，评估半月板发病机制中的炎症介质。
HE 染色结果显示，表面粗糙，组织疏松，胶原纤维厚度不一。关节滑液中 IL-1β和 IL-17 以及 MMP-13 的阳性率和 MMP-13mRNA 均呈 2 周到 8 周逐渐升高的趋势。与对照组相比，差异有统计学意义（P < 0.05）。
我们得出结论，在兔膝关节 ACL 部分损伤模型中，IL-1β和 IL-17 水平升高，MMP13 表达增加，导致半月板降解。
