Bjerrum O W, Bjerrum O J, Borregaard N
J Immunol. 1987 Jun 1;138(11):3913-7.
The subcellular localization of beta2-microglobulin (beta 2m) in human neutrophils was determined by an enzyme-linked immunosorbent assay on subcellular fractions obtained by Percoll density gradient centrifugation of neutrophils disrupted by nitrogen cavitation. The neutrophils were found to contain 160 ng beta 2m/mg protein. Approximately two-thirds co-located with the markers for specific granules and was released from intact cells during degranulation, whereas one-third of the beta 2m was located together with markers of the plasma membrane. This fraction was not further enriched during degranulation. These results indicate that beta 2m cannot be universally used as a plasma membrane marker as hitherto assumed, but beta 2m may serve as an indicator of neutrophil degranulation.
通过酶联免疫吸附测定法，对经氮空化破碎的中性粒细胞进行Percoll密度梯度离心获得的亚细胞组分进行检测，从而确定β2-微球蛋白（β2m）在人中性粒细胞中的亚细胞定位。发现中性粒细胞中含有160 ng β2m/mg蛋白质。大约三分之二的β2m与特异性颗粒的标志物共定位，并在脱颗粒过程中从完整细胞中释放出来，而三分之一的β2m与质膜标志物位于一起。该部分在脱颗粒过程中未进一步富集。这些结果表明，β2m不能像迄今所认为的那样普遍用作质膜标志物，但β2m可能作为中性粒细胞脱颗粒的一个指标。
