White K L, Anderson G B, Pashen R L, BonDurant R H
J Reprod Immunol. 1987 Jan;10(1):27-32. doi: 10.1016/0165-0378(87)90047-7.
Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% (50/57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.
采用间接免疫荧光法检测植入前绵羊胚胎上雄性特异性蛋白（H-Y抗原）的存在。从超数排卵的母羊中通过手术采集八细胞至囊胚期胚胎，分为荧光（H-Y阳性）或非荧光（H-Y阴性），然后将其移植到受体母羊或进行核型分析以确认胚胎性别。在八细胞至囊胚期胚胎上检测到了H-Y抗原。总体而言，分类为H-Y阳性或H-Y阴性的胚胎（八细胞至早期囊胚期）中，分别有88%（50/57）为雄性和雌性。经过H-Y抗原检测的胚胎移植后的存活率很高（63%），这支持将该方法与绵羊胚胎移植相结合，以产生已知胎儿性别的妊娠。
