Sasabe T, Kishida K, Kiritoshi A, Uni A, Manabe R
Jpn J Ophthalmol. 1986;30(4):367-75.
Rabbit lens epithelial cells have been cultured continuously for more than 24 months (200 generations) in monolayers. Their morphology resembled cobblestones when confluent and was spindle-shaped during growth. The doubling time was 40 hours. The cells were capable of colony formation and their plating efficiency was about 6%. They had immunoreactivity to antiserum to the crystalline-rich supernatant of rabbit lens homogenate. Therefore, it was concluded that they were a permanent cell line and they were named TOTL-86 cells.
兔晶状体上皮细胞已在单层培养中连续培养超过24个月（200代）。汇合时其形态类似于鹅卵石，生长期间呈纺锤形。倍增时间为40小时。这些细胞能够形成集落，其接种效率约为6%。它们对兔晶状体匀浆富含晶体的上清液抗血清具有免疫反应性。因此，得出结论，它们是一个永久细胞系，并被命名为TOTL-86细胞。
