与兔肝脏细胞色素P-450组成型形式相关的孕酮21-羟化酶活性的多样性。
Multiplicity of progesterone 21-hydroxylase activities associated with constitutive forms of rabbit liver cytochrome P-450.
作者信息
Sethumadhavan K, Senciall I R
出版信息
J Steroid Biochem. 1987 May;26(5):569-75. doi: 10.1016/0022-4731(87)90009-4.
Constitutive cytochromes P-450 have been solubilized from untreated outbred New Zealand White rabbit liver microsomes. Gradient phosphate buffer elution of DEAE-cellulose columns partially resolved six P-450 fractions. Progesterone 21-hydroxylase activity was reconstituted with several fractions and inhibited by an antibody towards P-450 Form 1. One fraction (LM3b) preferentially catalysed the 6 beta- and 16 alpha-hydroxylation of progesterone. SDS-PAGE indicated the presence of proteins with mobilities closely related to Form 1 in several fractions that were separated from this isozyme by DEAE-cellulose chromatography. These results suggest that several constitutive P-450 fractions may contribute to the regiospecific 21-hydroxylation of progesterone.
组成型细胞色素P-450已从未经处理的远交系新西兰白兔肝微粒体中溶解出来。用DEAE-纤维素柱进行梯度磷酸盐缓冲液洗脱，部分分离出六个P-450组分。几种组分重建了孕酮21-羟化酶活性，并被针对P-450 1型的抗体所抑制。其中一个组分（LM3b）优先催化孕酮的6β-和16α-羟化反应。SDS-PAGE表明，在通过DEAE-纤维素色谱法与该同工酶分离的几个组分中，存在迁移率与1型密切相关的蛋白质。这些结果表明，几种组成型P-450组分可能参与了孕酮区域特异性21-羟化反应。
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