Microbiology & Mycology Program, Biomedical Sciences Institute, School of Medicine, University of Chile, Santiago 8380453, Chile.
Laboratorio Clínico, Clínica Las Condes, RM, Santiago 8340518, Chile.
Future Microbiol. 2020 Aug;15:1123-1129. doi: 10.2217/fmb-2020-0068.
Antibiotic-dependent pathogenic bacteria are sporadically isolated from patients that received prolonged antibiotic treatments. Evolution of antibiotics dependence and its clinical implications are scarcely studied. A linezolid-dependent strain was isolated from a cystic fibrosis patient. A draft genome sequence was obtained and searched for known antibiotics resistance determinants and virulence factors. The genome was assembled into 79 contigs for a total of 2.83 Mbp. This strain is a sequence type 5 methicillin-resistant with a type I SCC cassette also conserving the Panton-Valentine leukocidin. The G2576T substitution, conferring linezolid resistance, was harbored by all five copies of the 23S rRNA. The linezolid-dependent strain is related to a strain circulating in Latin America that acquired a mutation conferring linezolid resistance.
从接受长期抗生素治疗的患者中偶尔会分离出依赖抗生素的病原菌。抗生素依赖性的进化及其临床意义研究甚少。从一名囊性纤维化患者中分离出一株依赖利奈唑胺的菌株。获得了该菌株的基因组草图序列，并对其进行了已知抗生素耐药决定因子和毒力因子的搜索。基因组被组装成 79 个 contigs，总长度为 2830 万 bp。该菌株为耐甲氧西林 5 型序列，具有 I 型 SCC 盒，还保留了杀白细胞素 Panton-Valentine。赋予利奈唑胺耐药性的 G2576T 取代存在于 23S rRNA 的所有 5 个拷贝中。依赖利奈唑胺的菌株与在拉丁美洲流行的一株菌株有关，该菌株获得了一个赋予利奈唑胺耐药性的突变。
