For prophylactic therapy, mice received an oral antibiotic cocktail followed by clindamycin injection, followed by probiotic administration (planktonic . biofilm state), followed by oral gavage. For treatment therapy, mice received antibiotics and first, followed by probiotic administration. Clinical sickness scores (CSS) and intestinal histologic injury scores (HIS) were assigned. In the model, CSS: 67% of untreated mice exposed to demonstrated CSS ≥ 6, which is consistent with infection (< .001 compared to unexposed mice). In mice treated with planktonic , 55% had a CSS ≥ 6, but only 19% of mice treated with in its biofilm state had CSS ≥ 6 (< .001). Mice receiving + DM-Maltose lost the least amount of weight compared to mice receiving saline ( = .004676) or to mice receiving (= .003185). HIS: 77% of untreated mice exposed to had HIS scores ≥4, which is consistent with infection. In mice treated with planktonic , 62% had HIS ≥4, but only 19% of mice treated with in its biofilm state had HIS ≥4. (< .001). Additionally, mice treated with in its biofilm state had better survival compared to untreated mice and to mice treated with planktonic ( ≤ 0.05). Similar findings for weight loss, CSS, HIS and survival were obtained for . A single dose of in its biofilm state reduces the severity and incidence of experimental infection when administered as both prophylactic and treatment therapy.