Center for Perinatal Research, The Research Institute at Nationwide Children's Hospital, Department of Pediatric Surgery, Nationwide Children's Hospital , Columbus, OH, USA.
Center for Microbial Pathogenesis, The Research Institute at Nationwide Children's Hospital , Columbus, OH, USA.
Gut Microbes. 2020 Nov 9;12(1):1814119. doi: 10.1080/19490976.2020.1814119.
For prophylactic therapy, mice received an oral antibiotic cocktail followed by clindamycin injection, followed by probiotic administration (planktonic . biofilm state), followed by oral gavage. For treatment therapy, mice received antibiotics and first, followed by probiotic administration. Clinical sickness scores (CSS) and intestinal histologic injury scores (HIS) were assigned. In the model, CSS: 67% of untreated mice exposed to demonstrated CSS ≥ 6, which is consistent with infection (< .001 compared to unexposed mice). In mice treated with planktonic , 55% had a CSS ≥ 6, but only 19% of mice treated with in its biofilm state had CSS ≥ 6 (< .001). Mice receiving + DM-Maltose lost the least amount of weight compared to mice receiving saline ( = .004676) or to mice receiving (= .003185). HIS: 77% of untreated mice exposed to had HIS scores ≥4, which is consistent with infection. In mice treated with planktonic , 62% had HIS ≥4, but only 19% of mice treated with in its biofilm state had HIS ≥4. (< .001). Additionally, mice treated with in its biofilm state had better survival compared to untreated mice and to mice treated with planktonic ( ≤ 0.05). Similar findings for weight loss, CSS, HIS and survival were obtained for . A single dose of in its biofilm state reduces the severity and incidence of experimental infection when administered as both prophylactic and treatment therapy.
对于预防治疗，小鼠接受口服抗生素鸡尾酒，然后注射克林霉素，然后给予益生菌（浮游生物、生物膜状态），然后口服灌胃。对于治疗治疗，小鼠接受抗生素和第一次治疗，然后给予益生菌。分配临床疾病评分（CSS）和肠道组织学损伤评分（HIS）。在该模型中，CSS：暴露于未处理的未处理的小鼠中有 67%表现出 CSS≥6，这与感染一致（<0.001与未暴露的小鼠相比）。在用浮游生物治疗的小鼠中，55%的小鼠 CSS≥6，但只有 19%的处于生物膜状态的小鼠 CSS≥6（<0.001）。与接受生理盐水（=0.004676）或接受（=0.003185）的小鼠相比，接受+DM-麦芽糖的小鼠体重减轻最少（=0.004676）。HIS：暴露于未处理的小鼠中有 77%的 HIS 评分≥4，这与感染一致。在用浮游生物治疗的小鼠中，62%的 HIS≥4，但只有 19%的处于生物膜状态的小鼠 HIS≥4。（<0.001）。此外，与未处理的小鼠和用浮游生物治疗的小鼠相比，用生物膜状态治疗的小鼠具有更好的存活率（≤0.05）。对于体重减轻、CSS、HIS 和存活，也得到了类似的发现。生物膜状态下的单剂量可降低实验感染的严重程度和发生率，无论是预防治疗还是治疗治疗。
