Department of Surgical Oncology, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, 318000, PR China.
Shanghai Engineering Research Center of Pharmaceutical Translation, Shanghai, 200000, PR China.
Future Oncol. 2021 Jan;17(3):279-289. doi: 10.2217/fon-2020-0415. Epub 2020 Sep 21.
The methylation and expression levels of in breast cancer were investigated and their effects on tumor cells were researched. Bioinformatics was employed to identify the target gene from TCGA database. Sodium bisulfite and decitabine were used for DNA modification and demethylation, and methylation-specific PCR and reverse transcriptase PCR were performed to assess methylation and expression levels. Tumor cell behaviors were assayed via several experiments. was hypermethylated, which caused its poor expression in breast cancer, whereas its overexpression significantly suppressed cancer cell proliferation, migration and invasion and induced apoptosis. negatively functions in cancer progression upon overexpression, indicating its potential as a therapeutic target for clinical treatment of breast cancer.
研究了在乳腺癌中的甲基化和表达水平及其对肿瘤细胞的影响。利用生物信息学从 TCGA 数据库中鉴定出靶基因。采用亚硫酸氢钠和地西他滨进行 DNA 修饰和去甲基化，甲基化特异性 PCR 和逆转录 PCR 评估 甲基化和表达水平。通过多种实验检测肿瘤细胞行为。在乳腺癌中呈高甲基化，导致其表达水平降低，而过表达则显著抑制癌细胞增殖、迁移和侵袭，并诱导细胞凋亡。过表达时在癌症进展中起负调控作用，表明其可能成为治疗乳腺癌的临床治疗靶点。
