Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo, Tokyo 113-0033, Japan.
Curr Opin Struct Biol. 2021 Feb;66:8-14. doi: 10.1016/j.sbi.2020.08.005. Epub 2020 Sep 18.
Centriole duplication occurs once per cell cycle. Since only a single daughter centriole is assembled adjacent to each mother centriole, symmetry around the mother centriole must be broken in the process of centriole duplication. Recent studies have established that Plk4, a master kinase for centriole duplication, can self-assemble into condensates, and have suggested that this Plk4 self-assembly is the key to symmetry breaking. Here, we present the current hypotheses for how Plk4 could break symmetry around the mother centriole via autonomous regulation. After this initial symmetry-breaking process, the ring-to-dot conversion of Plk4 around the mother centriole completes the selection of the site for procentriole formation. We also discuss how this dynamic transition contributes to the strict regulation of centriole duplication.
中心体复制发生在细胞周期的每一次分裂中。由于每个母中心体仅组装一个子中心体，因此在中心体复制过程中，母中心体周围的对称性必须被打破。最近的研究已经证实，中心体复制的主激酶 Plk4 可以自我组装成凝聚体，并表明这种 Plk4 自组装是打破对称性的关键。在这里，我们提出了 Plk4 如何通过自主调节打破母中心体周围对称性的当前假设。在这个初始的对称破坏过程之后，Plk4 围绕母中心体的环到点的转换完成了对前中心粒形成部位的选择。我们还讨论了这种动态转变如何有助于对中心体复制的严格调控。
