Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao 266071, China.
Laboratory for Marine Drugs and Bioproducts of Qingdao, National Laboratory for Marine Science and Technology, Qingdao 266000, China.
Mar Drugs. 2020 Sep 17;18(9):469. doi: 10.3390/md18090469.
Gut microbiota has a critical role in metabolic diseases, including type 2 diabetes mellitus (T2DM). 3-bromo-4,5-bis(2,3-dibromo-4,5-dihydroxybenzyl)-1,2-benzenediol (BDB) is a natural bromophenol isolated from marine red alga . Our latest research showed that BDB could alleviate T2DM in diabetic BKS db mice. To find out whether BDB modulates the composition of the gut microbiota during T2DM treatment, 24 BKS db diabetic mice were randomly grouped to receive BDB ( = 6), metformin = 6), or the vehicle ( = 6) for 7 weeks in a blinded manner. Non-diabetic BKS mice ( = 6) were used as normal control. Diabetic mice treated with BDB or metformin demonstrated significant reductions in fasting blood glucose (FBG) levels compared with the vehicle-treated mice in the 7th week. Pyrosequencing of the V3-V4 regions of the 16S rRNA gene revealed the changes of gut microbiota in response to BDB treatment. The result demonstrated short-chain acid (SCFA) producing bacteria Lachnospiraceae and Bacteroides were found to be significantly more abundant in the BDB and metformin treated group than the vehicle-treatment diabetic group. Remarkably, at the genus levels, elevated significantly in the BDB-treatment group. Metagenomic results indicated that BDB may alleviate the metabolic disorder of diabetic mice by promoting propanoate metabolism and inhibiting starch and sucrose metabolism, amino sugar and nucleotide sugar metabolism. In conclusion, our study suggests that the anti-diabetic effect of BDB is closely related to the modulating structure of gut microbiota and the improvement of functional metabolism genes of intestinal microorganisms.
肠道微生物群在代谢性疾病中起着关键作用，包括 2 型糖尿病（T2DM）。3-溴-4,5-双（2,3-二溴-4,5-二羟基苯甲基）-1,2-苯二酚（BDB）是一种从海洋红藻中分离出来的天然溴酚。我们的最新研究表明，BDB 可以缓解糖尿病 BKS db 小鼠的 T2DM。为了研究 BDB 是否在 T2DM 治疗过程中调节肠道微生物群的组成，将 24 只 BKS db 糖尿病小鼠随机分组，分别接受 BDB（ = 6）、二甲双胍（ = 6）或载体（ = 6）治疗，共 7 周，实验采用盲法。将非糖尿病 BKS 小鼠（ = 6）作为正常对照。第 7 周，与载体处理组相比，BDB 或二甲双胍处理的糖尿病小鼠的空腹血糖（FBG）水平显著降低。16S rRNA 基因 V3-V4 区的焦磷酸测序显示了肠道微生物群对 BDB 治疗的反应变化。结果表明，与载体处理的糖尿病组相比，短链酸（SCFA）产生菌lachnospiraceae 和 bacteroides 在 BDB 和二甲双胍处理组中更为丰富。值得注意的是，在 BDB 处理组中，丰度显著升高。宏基因组学结果表明，BDB 可能通过促进丙酸盐代谢和抑制淀粉和蔗糖代谢、氨基糖和核苷酸糖代谢，缓解糖尿病小鼠的代谢紊乱。综上所述，本研究表明，BDB 的抗糖尿病作用与其调节肠道微生物群结构和改善肠道微生物功能代谢基因密切相关。
