Yue Fangqian, Zou Yourui, Sun Shengyu, Wang Zhe, Huang Ling, Ma Hui
Clinical Medical College, Ningxia Medical University, Yinchuan 750004, China.
Department of Neurosurgery, General Hospital of Ningxia Medical University, Yinchuan 750004, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Aug;36(8):724-728.
Objective To detect the expression of choline kinase α (CHKA) in glioma and to further explore the effect of CHKA knockdown on the proliferation, invasion and migration of U87MG human glioma cells. Methods The mRNA expression of CHKA in high-grade gliomas and traumatic brain tissues was detected by real-time quantitative PCR. The expression of CHKA protein in high-grade gliomas and traumatic brain tissues was detected by Western blot analysis. The short hairpin RNA of CHKA (shCHKA) lentivirus and its control lentivirus (shNC) were constructed and used to infect U87MG glioma cells, which were then divided into the following three groups: shCHKA group, shNC group and blank control group. The proliferation of U87MG cells was examined by CCK-8 assay, the invasion ability of glioma cells was tested by Transwell invasion assay, and the migration ability of glioma cells was evaluated by scratch healing test. Results The CHKA mRNA and protein were highly expressed in glioma. Knockdown of CHKA gene inhibited the proliferation, invasion and migration of U87MG glioma cells. Conclusion The expression of CHKA in glioma tissue is significantly higher than that in the normal brain tissue, and knockdown of CHKA gene inhibits the proliferation, invasion and migration of glioma cells. It suggests that CHKA may be related to the occurrence and development of gliomas.
目的 检测胆碱激酶α（CHKA）在胶质瘤中的表达，并进一步探讨CHKA基因敲低对人U87MG胶质瘤细胞增殖、侵袭和迁移的影响。方法 采用实时定量PCR检测高级别胶质瘤组织和创伤性脑组织中CHKA的mRNA表达。采用蛋白质免疫印迹法检测高级别胶质瘤组织和创伤性脑组织中CHKA蛋白的表达。构建CHKA短发夹RNA（shCHKA）慢病毒及其对照慢病毒（shNC），并用于感染U87MG胶质瘤细胞，然后将其分为以下三组：shCHKA组、shNC组和空白对照组。采用CCK-8法检测U87MG细胞的增殖情况，采用Transwell侵袭实验检测胶质瘤细胞的侵袭能力，采用划痕愈合实验评估胶质瘤细胞的迁移能力。结果 CHKA的mRNA和蛋白在胶质瘤中高表达。敲低CHKA基因可抑制U87MG胶质瘤细胞的增殖、侵袭和迁移。结论 CHKA在胶质瘤组织中的表达明显高于正常脑组织，敲低CHKA基因可抑制胶质瘤细胞的增殖、侵袭和迁移。提示CHKA可能与胶质瘤的发生发展有关。
