Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico, Mexico.
Plan de Estudios Combinados en Medicina (PECEM), Facultad de Medicina, Universidad Nacional Autonoma de Mexico, Ciudad de Mexico, Mexico.
Folia Parasitol (Praha). 2020 Sep 17;67:2020.022. doi: 10.14411/fp.2020.022.
Taeniosis-cysticercosis caused by Taenia crassiceps (Zeder, 1800) is a useful experimental model for biomedical research, in substitution of Taenia solium Linnaeus, 1758, studied during decades to develop effective vaccination, novel anti-helminthic drugs and diagnostic tools. Cysticercosis in mouse (Mus musculus Linnaeus) is achieved by the larval subculturing of the Wake Forest University (WFU) strain of T. crassiceps. Golden hamster, Mesocricetus auratus (Waterhouse), has been shown to be the most suitable host for adult forms of parasite in experimental taeniosis. Metacestodes of T. crassiceps WFU multiply by budding without restrictions once inoculated into the mouse, while the number of tapeworms developed from these larvae in hamsters remains highly variable. Three objectives have been proposed to improve the infection of T. crassiceps WFU in hamsters: (1) to re-evaluate the need of immune suppression; (2) to investigate the advantage of infecting hamsters with metacestodes with in vitro protruded scolices; and (3) to compare a number of tapeworms developed from metacestodes subcultured in hamsters against those proliferated in mice. Our results demonstrated that when the evagination of murine metacestodes was high, the number of T. crassiceps WFU adults obtained from hamsters was also high. Immunosuppressive treatment remains relevant for this experimental rodent model. The hamster-to-hamster cysticercosis-taeniosis by T. crassiceps overcame the mouse-to-hamster model in the yield of adult specimens. In vitro scolex evagination and metacestode asexual proliferation in hamsters place this rodent model by T. crassiceps WFU as the most affordable experimental models with taeniids.
由多头带绦虫（Zeder，1800）引起的囊虫病是生物医学研究的一种有用的实验模型，它替代了已研究了几十年的猪带绦虫（Linnaeus，1758）来开发有效的疫苗、新型抗寄生虫药物和诊断工具。通过幼虫的次级培养，在小鼠（Mus musculus Linnaeus）中可以实现多头带绦虫的囊虫病。金黄仓鼠（Mesocricetus auratus （Waterhouse））已被证明是寄生虫成虫实验性多头带绦虫病的最合适宿主。在接种到小鼠后，WFU 株多头带绦虫的囊尾蚴通过出芽无限制地繁殖，而从这些幼虫在仓鼠中发育的绦虫数量变化很大。为了提高在仓鼠中感染多头带绦虫 WFU 的效果，提出了三个目标：（1）重新评估免疫抑制的必要性；（2）研究感染仓鼠时带有体外伸出的头节的囊尾蚴的优势；（3）比较在仓鼠中培养的囊尾蚴发育出的许多绦虫与在小鼠中增殖的那些绦虫。我们的结果表明，当鼠类囊尾蚴的出芽率高时，从仓鼠中获得的多头带绦虫 WFU 成虫数量也高。免疫抑制治疗仍然与这个实验性啮齿动物模型相关。多头带绦虫的仓鼠-仓鼠囊虫病-多头带绦虫病克服了鼠-仓鼠模型在成虫标本产量方面的不足。在仓鼠中体外头节出芽和囊尾蚴无性增殖使这种多头带绦虫 WFU 啮齿动物模型成为最具成本效益的多头带绦虫实验模型。
