儿童由 Epstein-Barr 病毒引起的传染性单核细胞增多症中异型单核细胞的性质和临床意义。

The Nature and Clinical Significance of Atypical Mononuclear Cells in Infectious Mononucleosis Caused by the Epstein-Barr Virus in Children.

机构信息

Dmitry Rogachev National Research Centre for Pediatric Hematology, Oncology and Immunology, Moscow, Russia.

Centre for Theoretical Problems of Physicochemical Pharmacology RAS, Moscow, Russia.

出版信息

J Infect Dis. 2021 May 28;223(10):1699-1706. doi: 10.1093/infdis/jiaa601.

DOI:10.1093/infdis/jiaa601

PMID:32959062

Abstract

Atypical mononuclear cells (AM) appear in significant numbers in peripheral blood of patients with Epstein-Barr virus (EBV)-associated infectious mononucleosis (IM). We investigated the number and lineage-specific clusters of differentiation (CD) expression of atypical mononuclear cells in 110 children with IM using the anti-CD antibody microarray for panning leukocytes by their surface markers prior to morphology examination. The AM population consisted primarily of CD8+ T cells with a small fraction (0%-2% of all lymphocytes) of CD19+ B lymphocytes. AM amount in children with mononucleosis caused by primary EBV infection was significantly higher than for IM caused by EBV reactivation or other viruses and constituted 1%-53% of all peripheral blood mononuclear cells compared to 0%-11% and 0%-8%, respectively. Children failing to recover from classic IM associated with primary EBV infection within 6 months had significantly lower percentage of CD8+ AM compared to patients with normal recovery rate.

摘要

异常单核细胞（AM）在外周血中大量出现于 Epstein-Barr 病毒（EBV）相关传染性单核细胞增多症（IM）患者。我们通过抗 CD 抗体微阵列对 110 例 IM 患儿的白细胞进行了表面标志物预筛选，研究了异常单核细胞的数量和谱系特异性分化（CD）表达簇。AM 群体主要由 CD8+T 细胞组成，一小部分（所有淋巴细胞的 0%-2%）为 CD19+B 淋巴细胞。由原发性 EBV 感染引起的单核细胞增多症患儿的 AM 数量明显高于 EBV 再激活或其他病毒引起的 IM，占所有外周血单个核细胞的 1%-53%，而分别为 0%-11%和 0%-8%。在 6 个月内未能从与原发性 EBV 感染相关的经典 IM 中恢复的患儿，其 CD8+AM 的百分比明显低于恢复正常的患者。