基于甲病毒的抗原制备。

Alphavirus-Based Antigen Preparation.

机构信息

PanTherapeutics, Lutry, Switzerland.

出版信息

Methods Mol Biol. 2021;2183:63-81. doi: 10.1007/978-1-0716-0795-4_6.

Abstract

Alphavirus-based vectors present an efficient approach for antigen preparation applied for vaccine development. Semliki Forest virus, Sindbis virus, and Venezuelan equine encephalitis virus have been engineered for high-level expression of antigens targeting infectious diseases and tumors. Alphaviruses possess a large application range as vectors can be delivered as naked RNA replicons, recombinant viral particles, and layered DNA plasmids. Immunization studies in animal models have provided protection against challenges with lethal doses of pathogenic infectious agents and tumor cells. So far, a limited number of clinical trials have been conducted for alphavirus vectors in humans.

摘要

基于甲病毒的载体为抗原制备提供了一种有效的方法，可应用于疫苗开发。Semliki Forest 病毒、辛德毕斯病毒和委内瑞拉马脑炎病毒已被工程化为针对传染病和肿瘤的高抗原表达水平。甲病毒作为载体具有广泛的应用范围，可以作为裸露的 RNA 复制子、重组病毒颗粒和分层 DNA 质粒进行传递。动物模型中的免疫研究提供了针对致死剂量致病性感染因子和肿瘤细胞的挑战的保护。到目前为止，已经在人类中进行了有限数量的甲病毒载体临床试验。