Faculty of Chemistry, University of Warsaw , Warsaw, Poland.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Nov;37(11):1888-1896. doi: 10.1080/19440049.2020.1818850. Epub 2020 Sep 22.
Solid-phase extraction (SPE) was carefully optimised for preconcentration of 2-4-dimethylaniline (2,4-DMA) and 2,4-dimethylformanilide (DMF) from honey samples. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used for the separation and quantification of these analytes. By applying the SPE procedure with 200 mg of OASIS HLB sorbent, the limits of detection achieved in honey samples can be lowered to 0.41 µg·kg for 2,4-DMA and 0.69 µg L for DMF. The proposed method achieves good recoveries (81.1-114%) and precision (RSD 1.07-4.05%, n = 3) for analysed honey sample spiked at two concentration levels 1 µg L and 2 µg L. The results demonstrated our method can be applied as a simple way for sample preparation of honey samples for determination of amitraz degradation products. In 1 out of 5 of the analysed Polish honey samples, the DMF residues exceed the maximum residual limits (0.2 mg·kg for total amitraz residues).
固相萃取（SPE）经过精心优化，可从蜂蜜样品中预浓缩 2-4-二甲基苯胺（2,4-DMA）和 2,4-二甲基甲酰胺（DMF）。采用液相色谱-串联质谱法（LC-MS/MS）对这些分析物进行分离和定量。通过应用 200 mg OASIS HLB 吸附剂的 SPE 程序，可以将蜂蜜样品中的检测限降低到 2,4-DMA 为 0.41 µg·kg，DMF 为 0.69 µg·L。所提出的方法在分析的蜂蜜样品中具有良好的回收率（81.1-114%）和精密度（RSD 1.07-4.05%，n=3），在两个浓度水平 1 µg·L 和 2 µg·L 进行了加标。结果表明，该方法可作为一种简单的方法，用于测定咪鲜胺降解产物的蜂蜜样品的制备。在分析的 5 个波兰蜂蜜样品中，有 1 个 DMF 残留超过最大残留限量（总咪鲜胺残留 0.2 mg·kg）。
