Lyotropic Chromonic Liquid Crystals and Their Impurities Reveal the Importance of the Position of Functional Groups in Self-Assembly.

We study the effect of purification and impurities on the self-assembly and phase behavior of lyotropic chromonic liquid crystals (LCLCs). LCLC molecules in water stack to form aggregates; then, the elongated nanoaggregates align to make liquid crystalline phases. Utilizing multiple experimental techniques, we unveil impurities in commercial Sunset Yellow FCF (SSY), a representative LCLC, and how the precipitation-based purification promotes the formation of the aggregates and mesophase. We further explore the roles of intrinsic impurities, i.e., byproducts of the SSY synthesis, whose molecular structures are almost identical to that of SSY but differ only in the number and position of sulfonate groups. Combining quantum chemical calculations of molecular structures and experimental investigation of aggregate structures and phase behavior, we propose that the impurities of the planar shapes behave as planar SSY, i.e., participating in aggregate formation, whereas the nonplanar one disrupts the nematic phase. These results highlight the critical roles of the impurities and deepen our understanding of self-assembled aggregates and their aligned mesophases.