Department of Laboratory Medicine, Korea University College of Medicine, Seoul, Republic of Korea; Department of Laboratory Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea.
Korean Collection for Oral Microbiology and Department of Oral Biochemistry, College of Dentistry, Chosun University, Gwangju, Republic of Korea.
J Microbiol Methods. 2021 Jan;180:106065. doi: 10.1016/j.mimet.2020.106065. Epub 2020 Sep 20.
Rapid identification of bacterial pathogens facilitates earlier optimization of antibiotic treatment and reduces morbidity and mortality in sepsis patients. The aim of this research was to design an in-house chemical-free method for direct bacterial identification in positive blood culture (BC) broths and to compare the performance of this method with that of the commercial Sepsityper® kit. The overall species identification rates for the in-house and Sepsityper methods were 88.4% and 85.8%, respectively (n = 190). Among 146 facultative anaerobes, 92.5% and 95.9% were identified to the species level using the in-house and Sepsityper methods, respectively. For 32 anaerobic bacteria, the in-house method showed a higher species identification rate (75.0%) than the Sepsityper method (53.1%). The in-house method correctly identified more Bacteroides species (100.0%) than the Sepsityper method (18.2%). Our novel in-house method and the Sepsityper method showed a high accuracy for direct bacterial identification in positive BC broths using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
快速鉴定细菌病原体有助于更早地优化抗生素治疗，并降低脓毒症患者的发病率和死亡率。本研究旨在设计一种用于直接鉴定阳性血培养（BC）肉汤中细菌的内部无化学方法，并将其性能与商业 Sepsityper 试剂盒进行比较。内部方法和 Sepsityper 方法的总体物种鉴定率分别为 88.4%和 85.8%（n=190）。在 146 株需氧菌中，分别使用内部方法和 Sepsityper 方法对 92.5%和 95.9%的菌株进行了种水平鉴定。对于 32 株厌氧菌，内部方法的种鉴定率（75.0%）高于 Sepsityper 方法（53.1%）。内部方法鉴定出的更多拟杆菌属物种（100.0%）也多于 Sepsityper 方法（18.2%）。使用基质辅助激光解吸/电离飞行时间质谱法，我们的新型内部方法和 Sepsityper 方法在直接鉴定阳性 BC 肉汤中的细菌方面具有很高的准确性。
