Department of Biological Science and Technology, I Shou University, Kaohsiung 82445, Taiwan.
Graduate School of Human Life Science, Osaka City University, Osaka 558-8585, Japan.
Molecules. 2020 Sep 19;25(18):4304. doi: 10.3390/molecules25184304.
Di--butyl phthalate (DBP) is an extensively used plasticizer. Most investigations on DBP have been concentrated on its environmental distribution and toxicity to humans. However, information on the effects of plasticizers on algal species is scarce. This study verified the impacts of endocrine disruptor di--butyl phthalate ester on microalga by approaches of proteomics and gene ontology. The algal acute biotoxicity results showed that the 24h-EC50 of DBP for was 4.95 mg L, which caused a decrease in the chlorophyll a content and an increase in the DBP concentration of . Proteomic analysis led to the identification of 1257 proteins. Sixty-one more proteins showed increased expression, compared to proteins with decreased expression. This result illustrates that exposure to DBP generally enhances protein expression in . GO annotation showed that both acetolactate synthase (ALS) and GDP-L-fucose synthase 2 (GER2) decreased more than 1.5-fold after exposure to DBP. These effects could inhibit both the valine biosynthetic process and the nucleotide-sugar metabolic process in . The results of this study demonstrate that DBP could inhibit growth and cause significant changes to the biosynthesis-relevant proteins in .
邻苯二甲酸二丁酯（DBP）是一种广泛使用的增塑剂。大多数关于 DBP 的研究都集中在其环境分布和对人类的毒性上。然而，关于增塑剂对藻类物种影响的信息却很少。本研究通过蛋白质组学和基因本体论的方法，验证了内分泌干扰物邻苯二甲酸二丁酯对微藻的影响。藻类急性生物毒性结果表明，DBP 对 的 24h-EC50 为 4.95mg/L，导致叶绿素 a 含量降低，DBP 浓度升高。蛋白质组学分析鉴定了 1257 种蛋白质。与表达下调的蛋白质相比，有 61 种蛋白质的表达增加。这一结果表明，DBP 的暴露通常会增强 中的蛋白质表达。GO 注释显示，暴露于 DBP 后，乙酰乳酸合酶（ALS）和 GDP-L-岩藻糖合酶 2（GER2）的表达均下降了 1.5 倍以上。这些作用可能会抑制 的缬氨酸生物合成过程和核苷酸糖代谢过程。本研究结果表明，DBP 可以抑制 的生长，并导致其与生物合成相关的蛋白质发生显著变化。
