Impacts of Endocrine Disruptor di--Butyl Phthalate Ester on Microalga Verified by Approaches of Proteomics and Gene Ontology.

Di--butyl phthalate (DBP) is an extensively used plasticizer. Most investigations on DBP have been concentrated on its environmental distribution and toxicity to humans. However, information on the effects of plasticizers on algal species is scarce. This study verified the impacts of endocrine disruptor di--butyl phthalate ester on microalga by approaches of proteomics and gene ontology. The algal acute biotoxicity results showed that the 24h-EC50 of DBP for was 4.95 mg L, which caused a decrease in the chlorophyll a content and an increase in the DBP concentration of . Proteomic analysis led to the identification of 1257 proteins. Sixty-one more proteins showed increased expression, compared to proteins with decreased expression. This result illustrates that exposure to DBP generally enhances protein expression in . GO annotation showed that both acetolactate synthase (ALS) and GDP-L-fucose synthase 2 (GER2) decreased more than 1.5-fold after exposure to DBP. These effects could inhibit both the valine biosynthetic process and the nucleotide-sugar metabolic process in . The results of this study demonstrate that DBP could inhibit growth and cause significant changes to the biosynthesis-relevant proteins in .