Cao Xuan-Di, Jien Shih-Hao, Yang Chu-Wen, Lin Yi-Hsuan, Liao Chien-Sen
Institute of Biotechnology and Chemical Engineering, I-Shou University, Kaohsiung 840203, Taiwan.
Department of Soil and Environmental Sciences, National Chung Hsing University, Taichung 402202, Taiwan.
Microorganisms. 2024 Jun 22;12(7):1265. doi: 10.3390/microorganisms12071265.
Di--butyl phthalate (DBP) is a prevalent phthalate ester widely used as a plasticizer, leading to its widespread presence in various environmental matrices. This study presents an innovative microbial immobilization strategy utilizing biochar, calcium alginate (alginate-Ca, (CHCaO)n), and waterborne polyurethane (WPU) composites to enhance the biodegradation efficiency of DBP. The results revealed that rice husk biochar, pyrolyzed at 300 °C, exhibits relatively safer and more stable physical and chemical properties, making it an effective immobilization matrix. Additionally, the optimal cultural conditions for in DBP biodegradation were identified as incubation at 30 °C and pH 7, with the supplementation of 0.15 g of yeast extract, 0.0625 g of glucose, and 1 CMC of Triton X-100. Algal biotoxicity results indicated a significant decrease in biotoxicity, as evidenced by an increase in chlorophyll content in following DBP removal from the culture medium. Finally, microbial community analysis demonstrated that encapsulating within alginate-Ca and WPU layers not only enhanced DBP degradation, but also prevented ecological competition from indigenous microorganisms. This novel approach showcases the potential of agricultural waste utilization and microbial immobilization techniques for the remediation of DBP-contaminated environments.
邻苯二甲酸二丁酯（DBP）是一种普遍存在的邻苯二甲酸酯，被广泛用作增塑剂，导致其在各种环境基质中广泛存在。本研究提出了一种创新的微生物固定化策略，利用生物炭、海藻酸钙（alginate-Ca，(CHCaO)n）和水性聚氨酯（WPU）复合材料来提高DBP的生物降解效率。结果表明，在300℃下热解的稻壳生物炭表现出相对更安全、更稳定的物理和化学性质，使其成为一种有效的固定化基质。此外，确定DBP生物降解的最佳培养条件为在30℃和pH 7下培养，并添加0.15 g酵母提取物、0.0625 g葡萄糖和1 CMC的 Triton X-100。藻类生物毒性结果表明生物毒性显著降低，从培养基中去除DBP后，叶绿素含量增加证明了这一点。最后，微生物群落分析表明，将其包裹在海藻酸钙和WPU层中不仅增强了DBP的降解，还防止了本地微生物的生态竞争。这种新方法展示了农业废弃物利用和微生物固定化技术在修复受DBP污染环境方面的潜力。
