Department of Pathology, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.
Department of Internal Medicine, College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan, Taiwan.
Leuk Lymphoma. 2021 Jan;62(1):76-85. doi: 10.1080/10428194.2020.1817438. Epub 2020 Sep 23.
Regulatory T-cells (Tregs) play an important role in cancer immunity but their prognostic impact is controversial in diffuse large B-cell lymphoma (DLBCL). Intratumoral Tregs in DLBCL ( = 70) were evaluated by double-stained CD25 and FOXP3 lymphocytes in formalin-fixed paraffin-embedded tissues, and correlated with clinicopathologic features. We found that increased numbers of intratumoral FOXP3+ lymphocytes (>2.4/HPF) and CD25 + FOXP3+ lymphocytes (>0.8/HPF) are favorable prognosticators ( = .004 and < .001, respectively) in DLBCL patients, along with age <70 years, stage I-II disease, normal serum LDH level and low IPI scores ( .001, .002, .002, and <.001, respectively). On multivariate analyses, a higher number of CD25 + FOXP3+ lymphocytes retained prognostic significance ( = .040). Interestingly, higher Treg infiltration correlated with increased infiltration by cytotoxic T-lymphocytes ( = 0.294, = .038) and nodal location ( = 0.390, = .004), but not with infiltration by CD123+ plasmacytoid dendritic cells, which were reported to induce Tregs with immune tolerance. Therefore, congruent with literature meta-analyses, higher intratumoral CD25 + FOXP3+ lymphocytes have a beneficial impact on DLBCL.
调节性 T 细胞（Tregs）在癌症免疫中发挥重要作用，但它们在弥漫性大 B 细胞淋巴瘤（DLBCL）中的预后影响存在争议。通过在福尔马林固定石蜡包埋组织中对 CD25 和 FOXP3 双染的淋巴细胞评估 DLBCL 中的肿瘤内 Tregs（ = 70），并将其与临床病理特征相关联。我们发现，肿瘤内 FOXP3+淋巴细胞（>2.4/高倍视野）和 CD25 + FOXP3+淋巴细胞（>0.8/高倍视野）数量增加是 DLBCL 患者的有利预后指标（分别为 = .004 和 < .001），同时年龄<70 岁、I-II 期疾病、正常血清 LDH 水平和低 IPI 评分（分别为 = .001、.002、.002 和 < .001）。在多变量分析中，较高的 CD25 + FOXP3+淋巴细胞数量保留了预后意义（ = .040）。有趣的是，较高的 Treg 浸润与细胞毒性 T 淋巴细胞浸润的增加相关（ = 0.294， = .038）和淋巴结位置相关（ = 0.390， = .004），但与 CD123+浆细胞样树突状细胞浸润无关，后者被报道可诱导具有免疫耐受的 Treg。因此，与文献荟萃分析一致，较高的肿瘤内 CD25 + FOXP3+淋巴细胞对 DLBCL 有有益的影响。
