秦柏清咳浓缩丸对感染后咳嗽大鼠 P 物质和中性内肽酶的影响。
Effect of Qinbai Qingfei Concentrated Pellets on substance P and neutral endopeptidase of rats with post-infectious cough.
机构信息
Department of Emergency, Nangang Branch of Heilongjiang Academy of Traditional Chinese Medicine, Harbin, 150001, China.
Department of Orthopedics, Heilongjiang Provincial Hospital, Harbin, 150036, China.
出版信息
BMC Complement Med Ther. 2020 Sep 22;20(1):289. doi: 10.1186/s12906-020-03081-5.
BACKGROUND
In recent years, it has been reported that Qinbai Qingfei Concentrated Pellet (QQCP) has the effect of relieving cough and reducing sputum. However, the therapeutic potentials of QQCP on post-infectious cough (PIC) rat models has not been elucidated. So the current study was aimed to scientifically validate the efficacy of QQCP in post infectious cough.
METHODS
All rats were exposed to sawdust and cigarette smokes for 10 days, and intratracheal lipopolysaccharide (LPS) and capsaicin aerosols. Rats were treated with QQCP at dose of 80, 160, 320 mg/kg. Cough frequency was monitored twice a day for 10 days after drug administration. Inflammatory cell infiltration was determined by ELISA. Meanwhile, the histopathology of lung tissue and bronchus in rats were evaluated by hematoxylin-eosin staining (H&E). Neurogenetic inflammation were measured by ELISA and qRT-PCR.
RESULTS
QQCP dose-dependently decreased the cough frequency and the release of pro-inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8, but exerted the opposite effects on the secretion of anti-inflammatory cytokines IL-10 and IL-13 in BALF and serum of PIC rats. The oxidative burden was effectively ameliorated in QQCP-treated PIC rats as there were declines in Malondialdehyde (MDA) content and increases in Superoxide dismutase (SOD) activity in the serum and lung tissue. In addition, QQCP blocked inflammatory cell infiltration into the lung as evidenced by the reduced number of total leukocytes and the portion of neutrophils in the broncho - alveolar lavage fluid (BALF) as well as the alleviated lung damage. Furthermore, QQCP considerable reversed the neurogenetic inflammation caused by PIC through elevating neutral endopeptidase (NEP) activity and reducing Substance P (SP) and Calcitonin gene related peptide (CGRP) expression in BALF, serum and lung tissue.
CONCLUSIONS
Our study indicated that QQCP demonstrated a protective role of PIC and may be a potential therapeutic target of PIC.
背景
近年来，有报道称秦贝清费浓缩丸（QQCP）具有止咳化痰的作用。然而，QQCP 对感染后咳嗽（PIC）大鼠模型的治疗潜力尚未阐明。因此，本研究旨在从科学上验证 QQCP 治疗感染后咳嗽的疗效。
方法
所有大鼠均暴露于木屑和香烟烟雾中 10 天，并经气管内给予脂多糖（LPS）和辣椒素气溶胶。大鼠给予 QQCP 剂量为 80、160、320mg/kg。给药后每天监测咳嗽频率两次，共 10 天。通过 ELISA 测定炎性细胞浸润。同时，通过苏木精-伊红（H&E）染色评估大鼠肺组织和支气管的组织病理学变化。通过 ELISA 和 qRT-PCR 测定神经遗传炎症。
结果
QQCP 呈剂量依赖性降低咳嗽频率和促炎细胞因子 TNF-α、IL-1β、IL-6 和 IL-8 的释放，但对 PIC 大鼠 BALF 和血清中抗炎细胞因子 IL-10 和 IL-13 的分泌产生相反的作用。QQCP 治疗 PIC 大鼠有效减轻氧化应激负担，表现为血清和肺组织丙二醛（MDA）含量降低，超氧化物歧化酶（SOD）活性升高。此外，QQCP 抑制炎性细胞浸润到肺组织，表现为支气管肺泡灌洗液（BALF）中总白细胞和中性粒细胞比例减少，肺损伤减轻。此外，QQCP 通过提高中性内肽酶（NEP）活性，降低 BALF、血清和肺组织中 P 物质（SP）和降钙素基因相关肽（CGRP）的表达，显著逆转了 PIC 引起的神经遗传炎症。
结论
本研究表明，QQCP 对 PIC 具有保护作用，可能是 PIC 的潜在治疗靶点。
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