School of Ecology and Environmental Science, Yunnan University, Kunming, 650504, China; National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, 230036, China.
National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, 230036, China.
Biochem Biophys Res Commun. 2021 Jun 18;558:196-201. doi: 10.1016/j.bbrc.2020.09.039. Epub 2020 Sep 19.
Phosphate transporters (PHTs) are well-known for their roles in phosphate uptake in plants. However, their actions in imparting plant growth in plants are still not so clear. In our previous study, we observed that maize PHT1 gene ZmPt9 plays a significant role in phosphate uptake. In this study, we further characterized ZmPt9 in response to low phosphate condition through ZmPt9 promoter inductive analysis by GUS staining and quantification. To elucidate the function of ZmPt9, we generated overexpression plant in Arabidopsis. ZmPt9 overexpressing Arabidopsis plants conferred small leaves and early flowering compared with the wild-type plants. In addition, ZmPt9 can complement the late flowering phenotype of Arabidopsis mutant pht1;2. The qRT-PCR analysis revealed that overexpression of ZmPt9 in Arabidopsis changed expression levels of some flowering-related genes. Further expressed detection of hormone related genes revealed that GA and auxin maybe the main determinant for growth influences of ZmPt9. In conclusion, these results suggest that apart from phosphate transport activity, ZmPt9 can be further exploited for improving crops growth.
磷酸盐转运蛋白（PHTs）在植物的磷酸盐吸收中起着重要的作用。然而，它们在赋予植物生长方面的作用还不是很清楚。在我们之前的研究中，我们观察到玉米 PHT1 基因 ZmPt9 在磷酸盐吸收中起着重要的作用。在本研究中，我们通过 GUS 染色和定量分析进一步研究了 ZmPt9 在低磷条件下的响应。为了阐明 ZmPt9 的功能，我们在拟南芥中生成了过表达植株。与野生型植物相比，ZmPt9 过表达的拟南芥植株具有较小的叶片和提前开花的特征。此外，ZmPt9 可以弥补拟南芥突变体 pht1;2 的开花晚的表型。qRT-PCR 分析显示，ZmPt9 在拟南芥中的过表达改变了一些与开花相关基因的表达水平。进一步检测激素相关基因的表达发现，GA 和生长素可能是 ZmPt9 对生长影响的主要决定因素。总之，这些结果表明，除了磷酸盐转运活性之外，ZmPt9 还可以进一步用于改善作物生长。
