Belisario M A, Carrano L, De Giulio A, Pecce R, Buonocore V
Toxicol Lett. 1987 May;36(3):233-41. doi: 10.1016/0378-4274(87)90191-3.
Non-induced and phenobarbital (PB) or methylcholanthrene (MC) pretreated rats were injected with 1-nitropyrene (1-NP). Mutagenic activity of urine and feces samples were compared by the Salmonella/microsome assay. The highest, indirect-acting mutagenicity was associated with urines from MC-induced rats; HPLC analysis of organic extracts of urine samples showed that the differences in mutagenic response can be ascribed to different amounts of hydroxy derivatives of N-acetylaminopyrene excreted. Monohydroxy derivatives of 1-NP, being detected in the HPLC profiles of urine from PB-induced rats only, could be responsible of the higher direct-acting mutagenic activity of these samples as compared to urine from non-induced or MC-induced rats. The excretion rate of aminopyrene, the main metabolite of 1-NP identified in rat feces samples, was not affected by inducer pretreatment.
对未诱导以及经苯巴比妥（PB）或甲基胆蒽（MC）预处理的大鼠注射1-硝基芘（1-NP）。通过沙门氏菌/微粒体试验比较尿液和粪便样本的诱变活性。最高的间接作用诱变性与MC诱导大鼠的尿液相关；尿液样本有机提取物的HPLC分析表明，诱变反应的差异可归因于排泄的N-乙酰氨基芘羟基衍生物的量不同。仅在PB诱导大鼠尿液的HPLC图谱中检测到的1-NP单羟基衍生物，可能是这些样本与未诱导或MC诱导大鼠尿液相比具有更高直接作用诱变活性的原因。在大鼠粪便样本中鉴定出的1-NP主要代谢物氨基芘的排泄率不受诱导剂预处理的影响。
