Ahmad Fayyaz, Bibi Shaheen, Kang Mincheol, Anees Mariam, Ansar Muhammad, Alam Muhammad Rizwan, Kim Sun Yeou, Wahedi Hussain Mustatab
Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, 45320 Islamabad, Pakistan.
College of Pharmacy, Gachon University, 191 Hambakmaero, Incheon, South Korea.
Iran J Basic Med Sci. 2020 Sep;23(9):1139-1145. doi: 10.22038/ijbms.2020.43706.10275.
Lapachone is a natural naphthoquinone-derived compound found in . It is well-known for its analgesic, anti-inflammatory, anti-microbial, diuretic, and anti-cancerous effects. However, the wound-healing effects of this compound are not known yet. The aim of this study was to investigate the wound healing activity of naphthoquinones (α-lapachone and β-lapachone) from .
Expression of Sirt3, migration-related proteins (Rac1, Cdc42, α-Pak) and angiogenesis-related protein of vascular endothelial growth factor (VEGF) was monitored using western blot analysis. Blood vessel formation and tissue development were monitored by angiogenesis assay and hematoxylin & eosin (H & E) staining, respectively on mouse skin tissue samples. Both α-lapachone and β-lapachone increased Sirt3 expression , but only β-lapachone increased Sirt3 expression
Both the compounds accelerated wound healing in cultured skin cells as well as mouse skin; however, β-lapachone was more effective at lower concentrations. Both of the compounds increased the expression of migration-related proteins both and . Similarly, α-lapachone and β-lapachone increased VEGF expression, tissue development and blood vessel formation in mouse skin.
These findings indicated that α-lapachone and β-lapachone are novel Sirt3 activators, and Sirt3 has a role in wound healing. Thus, Sirt3 and its regulators come out as a novel target and potential drug candidates, respectively in the important field of cutaneous wound healing.
拉帕醇是一种天然萘醌衍生化合物。它以其镇痛、抗炎、抗菌、利尿和抗癌作用而闻名。然而，这种化合物的伤口愈合作用尚不清楚。本研究的目的是研究从[具体来源未给出]中提取的萘醌（α-拉帕醇和β-拉帕醇）的伤口愈合活性。
使用蛋白质免疫印迹分析监测Sirt3、迁移相关蛋白（Rac1、Cdc42、α-Pak）和血管内皮生长因子（VEGF）的血管生成相关蛋白的表达。分别通过血管生成测定和苏木精与伊红（H&E）染色对小鼠皮肤组织样本监测血管形成和组织发育。α-拉帕醇和β-拉帕醇均增加Sirt3表达[此处表述不完整，原文可能有误]，但只有β-拉帕醇增加Sirt3表达[此处表述不完整，原文可能有误]
两种化合物在培养的皮肤细胞以及小鼠皮肤中均加速伤口愈合；然而，β-拉帕醇在较低浓度下更有效。两种化合物均增加[此处表述不完整，原文可能有误]迁移相关蛋白的表达。同样，α-拉帕醇和β-拉帕醇增加小鼠皮肤中VEGF表达、组织发育和血管形成。
这些发现表明α-拉帕醇和β-拉帕醇是新型Sirt3激活剂，且Sirt3在伤口愈合中起作用。因此，在皮肤伤口愈合这一重要领域，Sirt3及其调节剂分别成为新的靶点和潜在的候选药物。
