鉴定 DNA 复制调控因子 MCM 复合物在肝癌中的表达及其预后意义。
Identification of the DNA Replication Regulator MCM Complex Expression and Prognostic Significance in Hepatic Carcinoma.
机构信息
Department of Digestive Medical, The Affiliated Nanhua Hospital, University of South China, Hengyang 421002, China.
Department of Gastrointestinal Surgery, The Affiliated Nanhua Hospital, University of South China, Hengyang 421002, China.
出版信息
Biomed Res Int. 2020 Sep 9;2020:3574261. doi: 10.1155/2020/3574261. eCollection 2020.
BACKGROUND
The microliposome maintenance (MCM) complex, MCM2-7, is revealed to be involved in multiple cellular processes and plays a key role in the development and progression of human cancers. However, the MCM complex remains poorly elaborated in hepatic carcinoma (HCC).
METHODS
In the study, we found the mRNA and protein level by bioinformatics. We also explored the prognostic value, genetic alteration, interaction network, and functional enrichment of MCM2-7. The MCM expression and correlation among these MCMs in HCC cell lines were identified by western blot.
RESULTS
MCM2-7 was significantly increased in HCC tissues compared to normal liver tissues. The high level of MCM2-7 had a positive correlation with poor prognosis. However, MCM2-7 alterations were not correlated with poor OS. MCMs were both increased in HCC cell lines compared to the normal hepatocyte cell line. Furthermore, the positive correlation was found among MCMs in HCC cell lines.
CONCLUSIONS
The MCM complex was increased in HCC tissues and cell lines and negatively correlated with prognosis, which might be important biomarkers for HCC.
背景
微脂体维持（MCM）复合物 MCM2-7 被揭示参与多种细胞过程，并在人类癌症的发展和进展中发挥关键作用。然而，MCM 复合物在肝癌（HCC）中的作用仍不清楚。
方法
在这项研究中，我们通过生物信息学发现了 mRNA 和蛋白质水平。我们还探索了 MCM2-7 的预后价值、遗传改变、相互作用网络和功能富集。通过 Western blot 鉴定 HCC 细胞系中 MCM2-7 的表达和这些 MCM 之间的相关性。
结果
与正常肝组织相比，MCM2-7 在 HCC 组织中显著增加。高水平的 MCM2-7 与预后不良呈正相关。然而，MCM2-7 的改变与 OS 不良无关。与正常肝细胞系相比，HCC 细胞系中 MCMs 均增加。此外，在 HCC 细胞系中发现 MCMs 之间存在正相关。
结论
MCM 复合物在 HCC 组织和细胞系中增加，与预后呈负相关，可能是 HCC 的重要生物标志物。
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