长链非编码 RNA HMGA1P4 促进胃癌顺铂耐药。
LncRNA HMGA1P4 promotes cisplatin-resistance in gastric cancer.
机构信息
Department of Gastroenterology, The Second Affiliated Hospital of Dalian Medical University, Dalian, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Sep;24(17):8830-8836. doi: 10.26355/eurrev_202009_22822.
OBJECTIVE
Long non-coding RNA (lncRNA) HMGA1P4 has been previously reported to be upregulated in gastric cancer (GC). This study aims to investigate the role of HMGA1P4 in cisplatin (DDP)-resistant GC.
PATIENTS AND METHODS
HMGA1P4 levels in DDP-resistant GC tissues and cells were determined. Regulatory effects of HMGA1P4 on proliferative and apoptotic abilities in DDP-resistant GC cells and their parental cells were assessed. At last, expression levels of genes associated with multidrug-resistance (MDR) (MDR1, MRP1, mTOR and HIF-1α) and apoptosis (Bax, Bcl-2 and Caspase3) were determined in DDP-resistant GC cells.
RESULTS
Results revealed that HMGA1P4 was upregulated in DDP-resistant GC tissues and cells. Overexpression of HMGA1P4 stimulated proliferative rate and suppressed apoptosis in both DDP-resistant GC cells and their parental cells. Moreover, in DDP-resistant GC cells, overexpression of HMGA1P4 upregulated MDR-related genes and downregulated apoptosis-related genes.
CONCLUSIONS
HMGA1P4 is upregulated in DDP-resistant GC tissues and cells, and triggers the progression of DDP-resistance in GC.
目的
长链非编码 RNA（lncRNA）HMGA1P4 先前已被报道在胃癌（GC）中上调。本研究旨在探讨 HMGA1P4 在顺铂（DDP）耐药 GC 中的作用。
患者和方法
检测 DDP 耐药 GC 组织和细胞中的 HMGA1P4 水平。评估 HMGA1P4 对 DDP 耐药 GC 细胞及其亲本细胞增殖和凋亡能力的调节作用。最后，检测 DDP 耐药 GC 细胞中与多药耐药（MDR）（MDR1、MRP1、mTOR 和 HIF-1α）和凋亡（Bax、Bcl-2 和 Caspase3）相关的基因表达水平。
结果
结果表明，HMGA1P4 在 DDP 耐药 GC 组织和细胞中上调。HMGA1P4 的过表达刺激了 DDP 耐药 GC 细胞及其亲本细胞的增殖率，并抑制了凋亡。此外，在 DDP 耐药 GC 细胞中，HMGA1P4 的过表达上调了 MDR 相关基因，下调了凋亡相关基因。
结论
HMGA1P4 在 DDP 耐药 GC 组织和细胞中上调，并引发 GC 中 DDP 耐药的进展。
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