Li Jiayi, Shen Haokun, Zhao Zengjue, Cao Ding, Zeng Min, Cai Haiming, Wei Jiatian, Fan Qin, Deng Jinbo, Ming Feiping, Ma Miaopeng, Liang Qianyi, Jia Junhao, Zhang Shuxia, Zhang Linghua
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, Guangdong, China.
Appl Microbiol Biotechnol. 2020 Nov;104(21):9343-9361. doi: 10.1007/s00253-020-10896-2. Epub 2020 Sep 23.
The human body is almost always facing the oxidative stress caused by foodborne aldehydes such as glyoxal (GO) and methylglyoxal (MGO), 4-hydroxyhexenal (HHE), and 4-hydroxynonenal (HNE). When these aldehydes build up, they can cause a range of harm. However, a probiotic, Clostridium butyricum, can increase nuclear factor erythroid-2 related factor 2 (Nrf2) and may have the potential to relieve oxidative stress. If C. butyricum is indeed resistant to aldehydes, the advantages (accessibility, convenience, and safety) will be of great significance compared with drugs. Unfortunately, whether C. butyricum can play a role in alleviating toxic effects of foodborne aldehydes in the intestine (the first line of defense against food-derived toxin) was unclear. To investigate these, we measured the viability, ROS, autophagy, and inflammatory cytokine expression of Caco-2 which were co-cultured with C. butyricum and stimulated by the four aldehydes via Nrf2 pathway (Staphylococcus aureus and Enterococcus faecium as controls). Then, we explored the link among C. butyricum, NLRP6, and Nrf2 signaling pathways when facing the stimuli. In the present study, we demonstrated that Clostridium butyricum relieved the oxidative stress induced by the aldehydes in Caco-2. Most interestingly, we found a "complementary" relationship between NLRP6 and Nrf2 in C. butyricum treatment under aldehyde stress. Our research not only makes a contribution to the popularization of C. butyricum as a probiotic-rich food instead of medicines but also sheds new light on the application of subsequent microecological formulation of C. butyricum. KEY POINTS: • The adverse effects are caused in a dose-dependent manner by foodborne aldehydes. • Clostridium butyricum can significantly ameliorate oxidative stress. • There is a "complementary" relationship between the NLRP6 and Nrf2 signaling pathways. • Using Clostridium butyricum foods to alleviate oxidative stress shows great prospects.
人体几乎总是面临着由食源性醛类物质引起的氧化应激，如乙二醛（GO）、甲基乙二醛（MGO）、4-羟基己烯醛（HHE）和4-羟基壬烯醛（HNE）。当这些醛类物质积累时，会造成一系列危害。然而，一种益生菌——丁酸梭菌，能够增加核因子红细胞2相关因子2（Nrf2），并可能具有缓解氧化应激的潜力。如果丁酸梭菌确实对醛类物质具有抗性，那么与药物相比，其优势（易获取、方便和安全）将具有重要意义。不幸的是，丁酸梭菌是否能在减轻肠道（抵御食物毒素的第一道防线）中食源性醛类物质的毒性作用方面发挥作用尚不清楚。为了研究这些问题，我们测量了与丁酸梭菌共培养并通过Nrf2途径受到四种醛类物质刺激的Caco-2细胞的活力、活性氧（ROS）、自噬和炎性细胞因子表达（以金黄色葡萄球菌和粪肠球菌作为对照）。然后，我们探究了丁酸梭菌、NLRP6和Nrf2信号通路在面对刺激时的联系。在本研究中我们证明，丁酸梭菌可减轻醛类物质在Caco-2细胞中诱导的氧化应激。最有趣的是，我们发现在醛类应激下，丁酸梭菌处理过程中NLRP6和Nrf2之间存在一种“互补”关系。我们的研究不仅有助于将丁酸梭菌作为富含益生菌的食品而非药物进行推广，还为后续丁酸梭菌微生态制剂的应用提供了新的思路。要点：• 食源性醛类物质以剂量依赖的方式造成不良影响。• 丁酸梭菌可显著改善氧化应激。• NLRP6和Nrf2信号通路之间存在“互补”关系。• 使用丁酸梭菌食品减轻氧化应激具有广阔前景。
