Department of Chemistry, Fudan University, Shanghai 200438, China.
Department of Clinical Laboratory, Yangpu Hospital, Tongji University School of Medicine, Shanghai, 200090, China.
Chem Commun (Camb). 2020 Oct 22;56(84):12793-12796. doi: 10.1039/d0cc04881j.
A novel cascade signal amplification based on tyramide signal amplification (TSA) and surface-initiated enzymatic polymerization (SIEP) was first reported for the sensitive and template-free detection of colorectal cancer (CRC) exosomes. This assay exhibited 20.9-fold signal amplification with a low detection limit of 12.8 particles per μL. Furthermore, accurate and reproducible results were obtained for detecting exosomes in serum samples, suggesting its potential application in exosomes analysis and clinical diagnostics.
首次报道了一种基于酪胺信号放大（TSA）和表面引发酶聚合（SIEP）的级联信号放大用于灵敏、无需模板的结直肠癌细胞（CRC）外泌体检测。该方法的信号放大倍数为 20.9 倍，检测限低至 12.8 个/μL。此外，该方法在血清样本中外泌体检测中获得了准确且可重复的结果，表明其在外泌体分析和临床诊断中的潜在应用。
