Sydney Institute of Agriculture, Plant Breeding Institute, The University of Sydney, Narellan 2567, NSW, Australia.
Plant Dis. 2021 Apr;105(4):743-747. doi: 10.1094/PDIS-08-20-1735-SC. Epub 2021 Mar 1.
Improving resistance to barley leaf rust (caused by ) is an important breeding objective in most barley growing regions worldwide. The development and subsequent utilization of high-throughput PCR-based codominant molecular markers remains an effective approach to select genotypes with multiple effective resistance genes, permitting efficient gene deployment and stewardship. The genes and confer widely effective adult plant resistance (APR) to leaf rust, are common in European and Australian barley germplasm (often in combination), and act interactively to confer high levels of resistance. Here we report on the development and validation of codominant insertion-deletion (indel) based PCR markers that are highly predictive for the resistance alleles and (both referred to as and ).
提高大麦抗叶锈病（由引起）的能力是全球大多数大麦种植地区的重要育种目标。高通量基于 PCR 的共显性分子标记的开发和随后的利用仍然是选择具有多个有效抗性基因的基因型的有效方法，允许有效基因部署和管理。基因和赋予大麦对叶锈病的广泛有效成株期抗性（APR），在欧洲和澳大利亚大麦种质中很常见（通常组合在一起），并相互作用以赋予高水平的抗性。在这里，我们报告了共显性插入-缺失（indel）基于 PCR 标记的开发和验证，这些标记对抗性等位基因和（两者都称为和）具有高度预测性。
